Expression of LDL receptor, apolipoprotein B, apolipoprotein A-I and apolipoprotein A-IV mRNA in various mouse organs as determined by a novel RNA-excess solution hybridization assay》.pdfVIP

Biochimica et Biophysica Acta, 1090(1991)95-101 95 © 1991ElsevierSciencePublishersB.V.0167-4781/91/${)3.50 ADONIS 0167478191002043 BBAEXP~ 2 ~ Expression of LDL receptor, apolipoprotein B, apolipoprotein A-I and apolipoprotein A-IV mRNA in various mouse organs as determined by a novel RNA-excess solution hybridization assay R a i Ajit K. Srivastava, B a r b a r a A. Pfleger a n d G u s t a v S c h o n f e l d Dicision of Atherosclerosis and Lipid Research. Department of Internal Medicine, Washington Unirersi~ School of Medicine. St. Louis, MO (U.S.A.) (Received 13November 1990) Keywords: Solutionhybridizationassay:Lowdensitylipoproteinreceptor;ApolipoproteinB:ApolipoproteinA-i;Apolipoprotein A-IV;(Mouseorgan) We report expression of LDL receptor, apolipoprotein B (apoB), apolipoprotein A-! (apoA-l) and apolipoprotein A-IV (apoAIV) mRNA in various mouse organs. These mRNA were quantified by an RNA-excess solution hybridization assay. For preparing specific probes, we cloned cDNA fragments of rat LDL receptor, apoB and apoA-i and mouse almA-IV into the polylinher region of pGEM3Zf(+) and used the recombinant vectors for preparing azP-laheled cRNA probes as well as RNA standards using the T7 and SP6 promoters flanking the polylinher regions. Preparation of cRNA probes and RNA standards is faster and more convenient than pre