Protective immunity against Eimeria tenella infection in chickens induced by immunization with a recombinant C-terminal derivative of EtIMP1》.pdf

Veterinary Immunology and Immunopathology 162 (2014) 117–121 Contents lists available at ScienceDirect Veterinary Immunology and Immunopathology j o u r na l h o m e pa g e : w w w. elsev /locate/vet imm Research paper Protective immunity against Eimeria tenella infection in chickens induced by immunization with a recombinant C-terminal derivative of EtIMP1 Guangwen Yin a , Qian Lin a , Wenjun Wei a , Mei Qin b , Xianyong Liu b,c , Xun Suo b,c , Zhijian Huang a,∗ a Engineering Laboratory of Animal Pharmaceuticals, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China b National Animal Protozoa Laboratory College of Veterinary Medicine, China Agricultural University, Beijing 100193, China c Key Laboratory of Zoonosis, China Ministry of Agriculture College of Veterinary Medicine, China Agricultural University, Beijing 100193, China a r t i c l e i n f o a b s t r a c t Article history: Immune mapped protein-1 (IMP1) is a new protective protein in apicomplexan parasites, Received 16 July 2014 and exits in Eimeria tenella. Cloning and sequence analysis has predicted the antigen to be a Received in revised form 17 October 2014 novel membrane protein of apicomplexan parasites. In order to assess the immunogenicity Accepted 21 October 2014 of EtIMP1, a C-terminal derivative of EtIMP1 was expressed in a bacterial host system and was used to immunize chickens. The protective efficacy against a homologous challenge was Keywords: evaluated by body weight gains, lesion scores and fecal oocyst shedding. The results s