Proteomic profile of aminoglutethimide-induced apoptosis in HL-60 cells Role of myeloperoxidase and arylamine free radicals》.pdfVIP

Chemico-Biological Interactions 239 (2015) 129–138 Contents lists available at ScienceDirect Chemico-Biological Interactions journal homepage: www.elsevier.c om/locate/chembioint Proteomic profile of aminoglutethimide-induced apoptosis in HL-60 cells: Role of myeloperoxidase and arylamine free radicals Saifur R. Khan a,1, Argishti Baghdasarian a,1, Prarthna H. Nagar b, Richard Fahlman b, Paul Jurasz a, Karim Michail a, Naif Aljuhani a,c, Arno G. Siraki a,⇑ a Faculty of Pharmacy Pharmaceutical Sciences, University of Alberta, Edmonton, AB T6G 2E1, Canada b Department of Biochemistry, Faculty of Medicine Dentistry, University of Alberta, Edmonton, AB T6G 2E1, Canada c Pharmacology and Toxicology Department, Faculty of Pharmacy, Taibah University, Madinah, Saudi Arabia a r t i c l e i n f o a b s t r a c t Article history: In this study, the cellular effects resulting from the metabolism of aminoglutethimide by myeloperoxi- Received 10 September 2014 dase were investigated. Human promyelocytic leukemia (HL-60) cells were treated with aminog- Received in revised form 26 May 2015 lutethimide (AG), an arylamine drug that has a risk of adverse drug reactions, including drug-induced Accepted 18 June 2015 agranulocytosis. HL-60 cells contain abundant amounts of myeloperoxidase (MPO), a hemoprotein, Available online 20 June 2015 which catalyzes one-electron oxidation of arylamines using H2O2 as a cofactor. Previous studies have shown that arylamine m