PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells》.pdf

PRPS2 Expression Correlates with Sertoli-Cell Only Syndrome and Inhibits the Apoptosis of TM4 Sertoli Cells Bin Lei,* Bo Wan,* Jian Peng,* Yu Yang, Daojun Lv, Xuming Zhou, Fangpeng Shu, Fei Li, Liren Zhong, Huayan Wu and Xiangming Mao† From the Departments of Urology, Peking University Shenzhen Hospital (BL, BW, JP, YY, XM), Shenzhen and Nanfang Hospital, Southern Medical University (BL, BW, DL, XZ, FS, FL, LZ, HW, XM), Guangzhou, People’s Republic of China Purpose: Sertoli-cell only syndrome is one of the reasons for male infertility but Abbreviations its pathogenesis remains unclear. PRPS2, a subset of PRS, is reported to be a and Acronyms potential protein associated with Sertoli-cell only syndrome. In this study we further investigated the correlation between PRPS2 and Sertoli-cell only GAPDH ¼ glyceraldehyde-3- phosphate dehydrogenase syndrome, and evaluated the effect of PRPS2 expression on apoptosis of TM4 Sertoli cells. IHC ¼ immunohistochemistry Materials and Methods: PRPS2 expression was detected in patients with PBS ¼ phosphate buffered saline Sertoli-cell only syndrome and normal spermatogenesis, and in Sertoli-cell only PCR ¼ polymerase chain reaction syndrome mouse models by immunohistochemistry, quantitative reverse PRPS2 ¼ PRS 2 transcription-polymerase chain reaction and Western blot. PR