一株芹菜(Apium+graveolens+L.)软腐病菌Pectobacterium+carotovorum+subsp.odoriferum的特征.pdf

植物病理学报 SINICA ACTAPHYTOPATHOLOGICA 45(4)：418-424(2015) 1 doi：10．13926／j．cnki．apps。2015．04．01 一株芹菜(Apiumgraveolens carotovorumsu 王茜1，张宝海2，赵亮1，马荣才1，谢华H (1北京市农林科学院北京农业生物技术研究中心，北京100097；2北京市农林科学院蔬菜研究中心，北京100097) 摘要：从北京通州地区芹菜软腐病样中分离获得的菌株QC01在实验室条件下能浸软芹菜叶柄组织，并能引起温室种植的 试的P．carotoyorum 解柠檬酸盐和液化明胶：除常见碳源外，QC01还能降解仅．甲基葡糖苷、麦芽糖、山梨醇、阿拉伯糖和阿拉伯半乳聚糖。基于 16S II与已报道 rRNA基因完整序列系统发育关系表明，QCOI与其他Pco菌株聚集成明显的Pco类群。QCOI和CCItype 582 II和NB1892的16S 的Pco菌株JKI type carotovorum subsp．odoriferum引发芹菜软腐病。 关键词：芹菜；软腐病；软腐果胶杆菌 carotovorum strain CharacterizationofaPectobacteriumsubsp．odorife厂￡，mcausing softrotdeseaseof WANG celery(ApiumgraveolensL．) Qianl，ZHANGBao—hai2，ZHAO Hual Research Liang，MARong—cail，XIE(1BeijingAgro—BiotechnologyCenter，Beijing Research of and Vegetable Center，BeijingAcademyAgricultureForestrySciences，Beijing100097，China) sn‘ain isolatedfromsoftrotted of in could Abstract：The celery District，it QC01 samples BeijingTongzhou the causesoftrot maceratetissuesof vitroinoculatedunder conditions，and celerypetioles／n laboratory on invivo inthe showedthat wasa symptomscelery planted greenhouse．Morphologicalanalyses Qc01 Pectobacteriumwith withP．carotovoum Gram—negative，rod—shapedflagellum．QC01together subsp．odor@rum and could at370Candevenin7％NaCIcontainedmedium：couldutilize