七位三芎汤对高脂饲喂致大鼠动脉粥样硬化的影响.pdfVIP

摘 要 目的：通过动物实验探讨活血化瘀法经验方七味三芎汤（三七粉 3 克，川芎 10 克， 生地 12 克，白芍 10 克，当归 6 克，檀香 15 克，丹参30 克）对动脉粥样硬化的干预， 并初步探讨其作用的可能机制。 方法：在 50 只大鼠中随机选取 11 只作为空白对照组，饲喂普通饲料，给余下 39 只 正常SD大鼠饲喂高脂饲料21周后检查血脂，随机选取1只空白组大鼠、2只模型组 大鼠做胸腹主动脉病理切片，确定形成动脉粥样硬化模型。造模成功后按体重随机分 为模型组，七味三芎汤组，辛伐他汀组。七味三芎汤组灌胃给予水煎煮法提取的七味 三芎汤浓缩混悬液 15g/kg，辛伐他汀组灌胃给予辛伐他汀水溶液 7mg/kg。灌胃 10 周后禁食12小时眼眶静脉取血检测血清血脂，血清超氧化物歧化酶（SOD）和血清丙 二醛含量（MDA）；隔天乌拉坦全麻后腹腔采血检测血管性血友病因子（VWF），血小板 聚集率；颈总动脉放血处死后，取胸腹主动脉全段固定于福尔马林做病理切片，免疫 组化方法检测血管壁组织基质金属蛋白酶（MMP-2）。 结果：通过对动脉粥样硬化模型大鼠分别灌胃七味三芎汤及辛伐他汀 10 周后，与模 型组比较七味三芎汤组及辛伐他汀组血清超氧化物歧化酶升高，血清丙二醛降低，血 小板聚集率降低，血管性血友病因子含量有降低趋势，主动脉壁变薄，纤维病变减轻。 结论：七味三芎汤对 AS 有良好的治疗作用，并且可以通过抑制 MMP-2 的合成稳定易 损斑块。 关键词：七味三芎汤；动脉粥样硬化；SOD；MDA；VWF；MMP-2 The Qiwei sanxiong decoction interfer with atherosclerosis by greases fodder feeds with rat Abstract Objective: Discuss what the Qiwei sanxiong decoction of promoting blood circulation and removing blood stasis as a law experience method (3 grams pseudo-ginseng powder，10 grams of Ligusticum wallichii，12 grams of the dried rhizome of rehmannia，10 grams of root of herbaceous peony，6 grams of Chinese angelica，15 grams of sandalwood by the animal experiment，30 overcomes Dan Shen Root)interfering with atherosclerosis is through animal experiments， and first step discussing the possibility mechanism. Methods: The random chooses 11 contrasting a group as blank space , fodder feeds with average feed in 50 rats, give remaining regular 39 SD rats to raise the grease examining blood after feeding with high grease feed four months, the random chooses 3 rats making belly aorta pathological mechanism section , ascertain the model forming an atherosclerosis. According to weight, the random is model group , traditional Chinese medicine group , Western medicine group after making a model successfully mark. Traditional Chinese medicine irrigates the sto