《MKP-3 is associated with recurrence after curative resection of hepatocellular carcinoma.》.pdf

ChineseMedicalJournal2014,127(7) l2l1 — — Originalarticle HigherintratumorthanperitumorexpressionofDUSP6／MKP-3is associatedwithrecurrenceaftercurativeresectionofhepatocellular CarCm 0m a YangBo，TanYunshan，SunHuichuan，FanJia，TangZhaoyouandJiYuan Keywords：DUSP6／MKP-3,hepatocellularcarcinoma；immunohistochemistry,t~suemicroarray；prognosis BackgroundTheMAPKphosphatases(MKPs)areafamilyofdual—specificityphosphatases(DUSPs)thatcan dephosphofflatebothphosphothreonineandphOsphOtVrOsineresidues，thusinactivatingMAPK signaling．DUSP6 ；s acytoplasmicMKPthatcaninactivateERK．DUSP6hasbeenimplicatedinthedevelopmentofsometumors．Theaim ofthisresearchwastoinvestigatetheexpressionofDUSP6inhepatOcelluIarcarcinomafHCC)andthecorrelationof 亡)USP6withmitogen．activatedproteinkinases(MAPKs)，clinicopathoIogicalcharacleristics，andprognosis． Methods Tissuesfr0m 305patientswhohadundergonehepatectomyforHCCwasused inthisstudy．Theexpression ofDUSP6，P—ERK，P．JNK，andP．p38awasdeterminedusingtissuemicroarraysforimmunohistochemicalanalysis．The prognosticvalueofDUSP6andothercIinIc0palhOlOgicalfalctorswereevaluated． Results TheexpressionofDUSP6wassignificantlyhigherinthetumortissuewhencomparedtotheperitumoror normalJivertissue(P0．001)．TumorDUSP6expressionwassignificantlyassociatedwithdisease．freesurvivaI(DFS) (P=0．013)．TumorDUSP6expressionwasanindependentprognosticfactorforDFS(Hazardratio=1．635，P=0．006)． ConcIusions DUSP6 isoverexpressed intumortissuecomparedtoperitumorornormalIivertissue．Higher expressionofDUSP6intumortissue．thaninperitumortissue，isassociatedwiththerecurrenceaftercurativeresection OfHCC．andtherelativetumorDUSP6expressionhasgoodpowertopredicttherecurrenceofHCC． ChinMedJ2014：127f7)：1211-1217 U epatocellu