《Mouse A6-positive Hepatic Oval Cells Derived from Embryonic Stem Cells.》.pdf

JHuazhongUnivSciTechno1[MedSci] DOI10．1007／s11596—014—1223—2 34(1)：1·9，2014 M ouseA6一positiveHepaticOvalCellsDerivedfrom EmbryonicStem ． Cells。 Dong．zhiYIN(银东智)一，Ji．yeCAI(蔡继业)，Qi-changZHENG(郑启昌)，Zheng-weiCHEN(陈正为)， Jing．xianZHAOf肇静娴)，You．nengYUAN(袁又能)。 DepartmentofGeneralSurgery,HuangshiCentralHospital，Huangshi435000,China eDepartmentofGeneralSu~ery,UnionHospital,TongjiMeScalCollege,HuazhongUniversityofScienceandTechnology Wuhan430030,China CollegeofLifeScienceandTechnoloyg,JinanUniversity,Guangzhou510632,China 4DepartmentofMicrobioloyg andImmunoloyg,UniversiytofIllinois，CollegeofMedicine,Chicago，1L60612,USA ◎HuazhongUniversityofScienceandTechnologyandSpringer-VerlagBerlinHeidelberg2014 Summary：Ovalcellshaveapotentialtodifferentiateintoavarieyt ofcelllineagesincludinghepato— cytesandbiliaryepithelia．Severalmodelshavebeenestablishedtoactivatetheovalcellsbyincorpo． ratingavarietyoftoxinsandcarcinogens．aloneorcombinedwithsurgicaltreatment．Thosemodelsare obviouslynotsuitableforthestudyonhumanhel：Iaticovalcells．Itisnecessarytoestablishanew and efficientmodeltosutdythehumanhepaticovalcells．Inthissutdy,thehepatocytegrowhtfactor(HGF) andepidermalgrowthfactor(EGF)wereusedtoinducedifferentiationofmouseembryonicstem (ES) cellsintohepaticovalcells．Wefirstconfirmedthathepaticovalcellsderivedfrom EScells．whichare bipotential，doexistduringthecourseofmouseEScells’differentiationintohepaticparenchymalcells． RT．PCR andtransmissionelecrtonmicroscopywereappliedinthissutdy．TheratioofSca一1+／CD34+ cellssortedbyFACSintheinductionrgoupwasincreasedfrom day4andreachedthemaximum onthe day 8．whereasthatin the controlgroup remained a