《六种真菌诱导子对石斛兰“088”组培苗生长的影响（英文版）》.pdfVIP

Agricultural Science Technology, 2015, 16(2): 219-223 Copyright 訫 2015, lnformation lnstitute of HAAS. All rights reserved Molecular Biology and Tissue Culture Effects of Six Kinds of Fungal Elicitors on Growth of Dendrobium hybrida cultivar ‘088 ’ Tissue Culture Seedlings 1, 2, 3 1, 2 4 1, 2 * Jinhua CHEN , Cun WANG , Lei SHl , Junmei YlN 1. Tropical Crops Genetic Resources lnstitute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China; 2. Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Danzhou 571737, China; 3. Engineering Technology Research Center of Tropical Ornamental Plant Germplasm lnnovation and Utilization, Danzhou 571737, China; 4. Nanjing Forestry University, Nanjing 210000, China Abstract To optimize the technique of rapid propagation of Dendrobium hybrida 六 种 真 菌 诱 导 子 对 石 斛 兰 seedlings and to explore a hormone-free tissue culture method for D. hybrida, six “088”组培苗生长的影响 kinds of mycorrhizal fungi which were isolated from the wild orchids were made into fungal elicitors. These fungal elicitors were added into the DE medium with concen- 陈金花 1, 2, 3，王 存 1, 2，石 蕾 4 ，尹俊梅 1, 2* （1.中 trations of 40, 60 and 80 ml/L, respectively. After a 90-d culturing, the effects of 国热带农业科学院热带作物品种资源研究所 ， ‘ ’ fungal elicitors on the growth of D. hybrida cultivar 088 tissue culture seedlings 海南儋州 571737 ；2.农业部华南作物基因资源 were studied. The results showed that treatment 13 (T13) extremely significantly in- 与 种 质 创 制 重 点 实 验 室 ，海 南 儋 州 571737 ； creased the fresh weight, but other treatmen