荔枝高频率体细胞胚胎发生和植株再生研究.pdf

5.用成熟的体胚在1/2MS(改良MS卜蔗糖2%+琼脂6.8g/L的成苗培养基上诱导植株， 到目前为止，只有水东和妃子笑诱导出了植株，其中水东的出苗率为16.57%，妃子笑的出苗 率为17.77%0 6.对组培苗进行炼苗锻炼，经过实验可以看出，适宜的炼苗程序为:在温度281C、光 照为3000Lx的培养室内闭瓶炼苗5d-10d;同样的环境下开瓶炼苗2d-3d，同时在开瓶炼苗 时要逐步揭开封口膜;组培苗应移栽在泥炭土和珍珠岩 2:1的培养基质上;对移栽后的 一个月内进行严格环境调控和管理。 关键词:荔枝 体胚发生 细胞学 炼苗 胚性愈伤组织 StudiesonHighFrequencySomaticEmbryogenesisand RegenerationCultureinLitchi Abstract Litchi(LitchichinesisSonn)belongstoSapindaceaefamilyandoriginatedinChinaandisa famousgreenfurittreesinsouthofChina.Itsdifficulttoculturelitchiinvitro,therefore, developmentofbiotechnologyinlitchiislimited.Inthisresearch,antherofFeizhixiao, Shuidong,No.2ofHaigenandSanshanjianyeliwereusedasmaterialstostudyhowtoobtain embryogeniccallus,topreserveandinducesomaticembryo.Andthenestablishandimprove thehighrfequencysomaticembryogenesissystemandplantregenerationsystemoflitchi. Expecttofoundthebiotechnologictheory,technologyandbiotechnologybreedingetcoflitchi. Themainresearchresultsareasfollows: 1.Theembryogeniccallusofseveralanthersoflitchiwasinducted,obtainedembryogenic callusrfomFeizixiao,ShuidongandSanshanjianyeli,andobtainednon-embryogeniccallusfrom No.2ofHaigen.FoundthatthemediumofMS+2,4-D2.Omg几十NAAO.5mg/L+PVP500m叭+ Sucrose50g几adaptedtoinducecallusofantheroflitchi. 2.Theembryogeniccallusofdifferentspeciesoflitchiwasconserved.Foundthatthe mediumofMS+NAA3.Omg/L+BAI.Omg/L+Sucrose6%+Agar6.8% isthebesttokeepthe embryogeniccallus.Long-termpreservationcanbeachievedbycombinedthemediumof MS+NAA3.Omg/L+BAI.Omg几+Sucrose6%+Agar6.8% andMSalternatively.Duringthe culturetheliquidmediumofMSshouldbeusedseveraltimes.Usingthemethod,the embryogeniccallusofShuidongandFeizixiaowasnotaffected.Butthenon-embryogeniccallus ofNo.2ofHaigenwasfoundeasytoturntoembryogeniccallus. 3.Themediumsthatadaptedtosomaticinductionoflitchiembryogeniccalluswere selectedbyusingtheorthogonalexperimentdesign.InthemediumofMS+BAO.5mg/L+ KTO.5mg/