《光照对黄芩种子萌发及代谢的影响（英文版）》.pdfVIP

GerminationCharacteristicsandSecondary MetabolismRegulationofScutellariabaicalensis GeorgiSeedsunderDifferentLightIntensities ZhaoweiSUN1,JinhuaLIU1,JiaLI2,YongqingZHANG2 1.JiningInstituteforDrugControl,Jining272000,China 2.ShandongUniversityofTraditionalChineseMedicine,Jinan250355,China [Objective]Thisstudyaimedtoinvestigatetheprimaryandsecondary 光照对黄芩种子萌发及代谢的 metabolismsduringthegerminationofScutellariabaicalensisGeorgiseedsunderdif 影响 ferentlightintensities.[Method]TheactivitiesofCHL,solublesugar,PAL,C4Hand CHSweredeterminedwithultravioletspectrophotometry.Thesecondarymetabolites 孙兆伟1刘金花1李佳2张永清2 1.济宁 weredetectedbyHighPerformanceLiquidChromatography(HPLC).[Result]There 市药品检验所，山东济宁2720002.山东中医 suitsindicatethatthegerminationofScutellariabaicalensisGeorgiseedsisnotsen 药大学，山东济南250355 sitivetolightandtheseedlingswereverysensitivetolight.TheCHL,solublesug ar,PAL,C4-HandCHScontinuouslyincreasedwithlightintensity.Thecontentof [目 的 ]了 解 光 照 强 度 在 黄 芩 secondarymetabolitesalsoincreased. [Conclusion]Lightincreasedtheformationof ScutellariabaicalensisGeorgi种子萌发过程中 leafphotosyntheticpigment,therebyaffectingtheprimarymetabolites.Theactivities 的初生与次生代谢规律。[方法]光合色素、可 ofPAL,C4HandCHSsignificantlyincreasedwiththedevelopmentoflightintensity. 溶性糖、苯丙氨酸解氨酶(PAL)、肉桂酸-4-羟 Finallythesecondarymetabolitesofmedicinalplantsincreasedsharply.Therefore, 化酶和查尔酮合成酶(CHS)活性测定采用紫 thequalityofScutellariabaicalensisGeorgimaterialscanbeimprovedbyincreasing thelightintensitymoderately. 外分光光度法。次生代谢物质含量测定采用高 Lightintens