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《DNA barcoding of Gaultheria L.in China (Ericaceae Vaccinioideae)》.pdf
Journal andEvolution
ofSystematics 49(5):411_424(2011)
ResearchArticle
DNA ofGaultheriaL.in
barcoding
1HeRENt1,2LuLUt WANG+1一De.ZhuLI
1,2Hong
650204,China)
650204,China)
Kunming
AbstractFourDNA loci nuclearinternaltranscribed
lOCUS
barcodingloci.chloroplastrbcL,matK。trnH-psbA,and
testedfortheaccuratediscriruinationof of
theChinese Gaultheria
spacer(ITS),were species byusingintraspecific
and rank tree—based includedl86
signedtest.and
interspecificpairwise尸.distance.Wilcoxon analyses.Thisstudy
individuals行om89 30 locusmarkersshowedlevels
populationsrepresentingspecies.Fora11individuals.single high
butwereineffectivefor chainreaction and
ofsequencinguniversality speciesresolvability.Polymeraseamplification
wereSuccessfulforallfourloci.BothJTSandrr/atKshowed levelsof
sequencing significantlyhigher interspecific
delimitationthanrbcLand combinationofmatKandITSwasthemoste疖cientDNAbarcode
species trnH-psbA.A
allstudied donot an candidatebarcodeforChinese
among regions。however,theyrepresentappropriate Gaultheria,
ll were
which outof30 canbe rbcL。matK,and
by only speciesseparat
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