《Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii》.pdfVIP

of and ChineseJournalOceanologyLimnology 、bI．26 No．3，E242—247，2008 DOI：10．1007／s00343·008-0242-x Efficient of fluorescent expressiongreen protein(GFP) mediatedachimeric inChlamydomonas by promoter · …4 reinharattt币 wU Jinxia(吴锦霞)，HU Zhangli(胡章立)料，WANGChaogang(王潮岗)， LI Shuangfei(黎X2Z)，LEIAnping(雷安平) Institute and Sciences，Shenzhen ofEcologyEnvironment,CollegeofLife University,Shenzhen，518060,China Received June Dec．13，2006；revisionaccepted15，2007 To Abstract the of in reinhardtii．a ireproveexpressionef!ficiencyexogenousgenesChlamydomonas efficient vectorwasconstructed．Greenfluorescent inC high expression protein(GFP)wasexpressed and reinhardtiiunderthecontrol and oftransformation ofpromoters：RBCs2HsP70A．RBCs2．Efficiency were between strainTran—Iand two expressioncompared transgenicalgae：RBC蛇mediated mediated could the HSP70A．RBCs2strainTran．II．Resultsshowt呱HsP70A．R8cS2 improvegreatly ofRBC蛇．andthe ofGFPin transformationefficiencybyapproximatelyeightfold expressionefficiency Tran．11wasatleastdoubleofthatinTran．I．Inaddition．athreefoldincreascofGFPinTran．11was inducedheatshockat400C．AuoftheresultsdemonstratedthatHsP70A-RBCS2wasmoreefficient by thallRBcs2in lnC，reinhardtii． expressingexogenousgene fluorescent efficiency；greenprotein(GFP)；HSP70A—RBCS2；RBCS2 Keyword：expression transformation