《GHR和IGF-IR在济宁青山羊子宫内分布和表达的发育性变化 Developmental Changes of the Distribution of GHR and IGF-IR and the Expression of Its mRNA in the Uterus of Jining Grey Goats》.pdfVIP
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《GHR和IGF-IR在济宁青山羊子宫内分布和表达的发育性变化 Developmental Changes of the Distribution of GHR and IGF-IR and the Expression of Its mRNA in the Uterus of Jining Grey Goats》.pdf
畜牧兽医学报2013,44(9):1392—1399
Ac£n、龙抛ri咒nrinP£Zoo£Pc矗恕ic口Si咒ic口
doi:10.11843/j.issn.0366—6964.2013.09.009
GHR和IGF—IR在济宁青山羊子宫内分布和
表达的发育性变化
同海妮1’2,白 淑1’2,王树迎1’弘,黄丽波1一,石运芝1’2,侯衍猛1’2
(1.山东农业大学动物科技学院,泰安271018;2.山东省动物生物技术与疫病防治重点实验室,泰安271018)
摘 要:旨在探讨济宁青山羊生后发育过程中生长激素受体(Growthhormone,GHR)和胰岛素样生长因子I受体
(Insulin-like
growth
GHR
mRNA和1GF—IR
明,GHR和IGF—IR免疫阳性染色主要见于子宫腺管上皮、基质、血管内皮和子宫平滑肌细胞的胞质中,偶见胞核
GHR和IGF_IR在子宫的生长发育过程中起着重要作用,且初情期和性成熟期可能是济宁青山羊生后子宫发育最
快的时期。
关键词:济宁青山羊;子宫;GHR;IGF_IR;免疫组化法;qRT-PCR
中图分类号:S827;S811.2文献标志码:A 文章编号:0366—6964(2013)09—1392—08
oftheDistributionofGHRandIGF-IRandthe
DeVelopmentalChanges
ofItsmRNAintheUterusof Goats
Expression JiningGrey
TONGHai-nil~,BAIShul”,ⅥANG Li_b01”,
Shu-yin91’2‘,HUANG
SHI
yu”zhil~,HOUYan-men91’2
(1.GDZZPggo厂A孢西7znZSfiP,zcP口咒d五c矗咒oZogy,S矗口咒do咒gAgric“Z£“raZUhi伽,芍i£y,了ai’n咒
P7‘o口西2ci口Z n咒d
271018,C矗i咒口;2.S矗n72do咒gKPyL口60rn£oryo厂A咒i7撇ZBio£Pc矗咒oZogy
Di5阻se
CD砌.0Z口蒯P他优删。孢,而i’彻271018,吼i触)
the ofGHRmRNAand
thelocalizationofGHRandIGF—IRand
Abstract:Tostudy expression
IGF—IRmRNAinuterusof Real—timefloures—
JiningGreygoatsduringpostnataldevelopment.
cence PCRwasusedtodetectthe ofGHRmRNAandIGF—IR
quantitative expressionpattern
mRNA,andSABCmethodwas toresearchthedistributionof cellsofGHRand
applied positive
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