《Identification of species within Tetrastigma (Miq.) Planch.(Vitaceae) based on DNA barcoding techniques》.pdfVIP
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《Identification of species within Tetrastigma (Miq.) Planch.(Vitaceae) based on DNA barcoding techniques》.pdf
Journal andEvolution
ofSystematics 49(3):237-245(2011)
ResearchArticle
Identificationof within
species
onDNA
(Vitaceae)basedbarcodingtechniques
1,2Yuan.MiaoFW1,2Wei.Mei FU4
JIANG撑1,2Cheng—Xin
Hangzhou310058,China)
Gene
and and SciencesandConservation Resources
EvolutionaryBotanyBiodiversity,InstituteofPlant Centerfor ofEndangered
2(LaboratoryofSystematic
玳ldt承.ZhejiangUniversity,Hangzhou310058,China)
Abstraet of beenusedasmedicinalin
ManyspeciesTetrastigma(Miq.、Planch.(Vitaceae)have10ng plants
someare dueto adulterantsareoftenaddedtotraditionalChinese
China,and endangeredoverexploitation.Although
medicines.thereisnoreliableor methodfor them.Inthis usedfourmarkers
practical identifying study,we
internaltranscribed DNAbarcodestotesttheir to
(rbcL。matK,trnH-psbA,andspacer『ITS])as abilitydistinguish
barcode
of resultsindicatedthatthebest wasITS.whichshowed
speciesTetrastigma.The significantinter-specific
thusits asaDNAbarcodefor loci a
geneticvariability,andpotential identifyingTetrastigma.Multipleprovided
to than loci.WerecommendthecombinedrbcL+matK+ITSbarcode
greaterabilitydistinguishspeciessingle using
forthe treesfromeachbarcodewere the
genus.Phyloge
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