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《Purification and Characterization of a Proteolytic Enzyme from Fig Latex》.pdf
online
Availableat
CHEM.RES.CHINESEUNIVERSITIES
.:7,
2008,24(3),348—352
ArticleID
1005··9040(2008)·-03-348·-05
PurificationandCharacterizationofaProteolytic
fromLatex
EnzymeFig
HUANGHe-zhi,ZHANG Shuo,
Lu,QU Lei,DUShan-shan,YANG
HAO andWANG
Dong-yun Xiao.ping+
Laboratory ofEducational
Key ofMolecularEnzymology&EngineeringofMinistry
Jilin 130021.户尼China
University,Changchun
all in as isof in
AbstractFicinis of Ficus such latex.It significance
importantcomponentplants family fig special
medicineand becauseitexhibits awide of and values.Inthis
industry activitythroughoutrangetemperaturepH work,
we a officinfromthelatex of the ofthe
trees,andpropertiespurified
purifiedcomponent homogeneityShandongfig
ficinwerestudied.Thecurrent revealedthat metalionswereabletoinhibitficin,while
findings heavy DTT,L—cysteine,
was officinat65。Cwas than1
and口.MEwerefoundto ficin alsoobservedthatthehalflife
promoteactivity.It longer
h forcasein was be showsthatthis
andtheMichaelis
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