《一株新基因型猪圆环病毒II型的鉴定 Identification of a Novel Genotype in Porcine Circovirus Type II》.pdfVIP

中国农学通报 2013,29(11):34-38 Chinese Agricultural Science Bulletin 一株新基因型猪圆环病毒II型的鉴定 1 1 1 2 1,2 2 谢俊岭 ，陈晓平 ，孟凡伟 ，王保有 ，周双海 ，王志军 1 2 （北京农学院动物科学技术学院，北京102206；天津市宁河原种猪场，天津301504） 摘 要：为了解新近出现的猪圆环病毒II 型(PCV2)的基因变异及其基因型信息。用PCR技术从新近发 生断奶后多系统消耗综合征的病猪组织中扩增和克隆出一株PCV2 全基因组序列，并进行了遗传变异 分析。结果显示：该PCV2毒株基因组全长为1767 bp，与部分其他PCV2毒株的核苷酸同源性达94.0% 以上，并显示出一定的地域、时间相关性。病毒ORF2 基因系统发育进化分析显示：该PCV2毒株与已有 PCV2 基因型的遗传距离均大于0.035 ，因而被划入到一个新的PCV2 基因型，暂命名为PCV2d，属于在 中国新近出现的一种PCV2基因型。研究结果表明，克隆鉴定了一株新基因型PCV2。 关键词：猪圆环病毒II 型；基因变异；进化分析；基因型 中图分类号：S852.659.2 文献标志码：A 论文编号：2012-2950 Identification of a Novel Genotype in Porcine Circovirus Type II Xie Junling1 1 1 2 1,2 2 ,Chen Xiaoping , Meng Fanwei , Wang Baoyou , Zhou Shuanghai , Wang Zhijun 1 ( SchoolofAnimalScienceandTechnology,BeijingUniversityofAgriculture, Beijing 102206; 2 TianjinNinghePrimaryPigBreedingFarm, Tianjin 301504) Abstract: The aim of the present study was to investigate the genetic variation and genotype information of a newly emerging Porcine Circovirus Type 2 (PCV2) strain. The complete genome sequence of one PCV2 strain was amplified and cloned from tissues of pig suffered newly from postweaning multisystemic wasting syndrome by PCR assay. Then, the genetic variation of several PCV2 strains was analyzed. The results showed that the complete genome of this cloned PCV2 strain was 1767 base pair in size, and shared more than 94.0% nucleotide identity with several other PCV2 strains. Moreov