《A New Cytotoxic Sterol Produced By an Endophytic Fungus from Castaniopsis Fissa at the Couth China Sea Coast》.pdfVIP

Chinese Chemical Letters Vol. 15, No. 4, pp 419-422, 2004 419 /journal/ccl.html A New Cytotoxic Sterol Produced by an Endophytic Fungus from Castaniopsis fissa at the South China Sea Coast 1 1 2 2 Hou Jin LI , Yong Cheng LIN *, L. L. P . VRIJMOED , E. B. G. JONES 1 Department of Applied Chemistry, Zhongshan University, Guangzhou 510275 2 Department of Biology and Chemistry, City University of Hong Kong, Hong Kong Abstract: A new sterol, ergosta-8(9),22-diene-3,5,6,7-tetraol(3â,5á,6â,7á,22E)(A) together with three known sterols: 3â, 5á,6â-trihydroxyergosta-7,22-diene (B), 3â-hydroxy-5á,8á - epidioxyer- gosta-6,22-diene (C) and ergosterol (D) were isolated from the mycelia of an unidentified endophytic fungus separated from Castaniopsis fissa (chestnut tree). Compound A exhibited potent selective cytotoxicity against Bel-7402, NCI4460 and L-02 cell lines with IC50 values 8.445, 5.03, 13.621 g/mL, respectively. Keywords: Endophytic fungus, sterols, ergosta-8(9), 22-diene-3,5,6,7-tetraol (3â,5á,6â,7á,22E), cytotoxicity. Endophytes are defined as fungi colonizing healthy tissue plant without overt symptoms or no apparent injury to the host. As the structurally novel and biologically active secondary metabolites possessing rich source, the endophytic fungi have gained increased attention