《Induction of HLA-G expression in a melanoma cell line OCM-1A following the treatment with 5-aza-2#39;-deoxycytidine》.pdfVIP

ofHLA·G inamelanomacelllineOCM·1A Induction expression with thetreatment 5-aza-2-deoxycytidine WeiHua Fen FERRONE2 YANl’2。，AiLINl，ChienCHANG2，Soldano Chung Cente，；WenzhouMedical Taizhou 1Laboratory AffiliatedHospital，Linhai317000,China College Institute，Buffalo，NY14263．USA 2DepartmentofImmunology，Roswell砌旭Cancer ABSTRACT Thenon—classicalHLAclassI HLA—GisanimmunemodulatorwhichinhibitsthefunctionsofT antigen Dendritic in cells themwith cells．andthe cellsfDC)．Asaresult，HLA—G amecha- expressionmalignantmayprovide nismto theimmunesurveillance．Inmelanoma．HLA—G hasbeenfoundin30％of escape antigenexpression butinlessnlanl％ofestablished removedlesions G invivoandfnvitroisthattheHLA．G is innlelanomacellsinvitro．Totest expression geneepigeneficallyrepressed melanomacellfineOCM一1AwiththeDNA 5一 this treated血eHLA．G inhibitor hypothesis，we negative methyltransferase whetherHLA—G canberestored．Ourdata that aza一2-deoxycytidiner5一AC)andanalyzed expression stronglysuggest asaresultof withina 220 of HLA-Gissilenced CpG region hypermethylation5’regulatoryencompassingbpupstream thestartcodon．Aftertreatment．皿A—GmRNA was increased．Westernblotandflow expressiond