《Preparation of Adenovirus 5 E1A Polyclonal Antibody》.pdfVIP

CHEM. RES. CHINESE UNIVERSITIES2009, 25(6), 891894 Preparation of Adenovirus 5 EIA Polyclonal Antibody 1,2 2 1 1 1* SHEN Oiang , MENG Fan-kai , YU Yi , ZHANG Yu and RUO Oi 1. Department Of NeurOsurgery, First HOspital Of Jilin University, Changchun 130021, P. R. China 2. Department Of NeurOsurgery, the PeOple s HOspital Of Jilin PrOvince, Changchun 130021, P. R. China Abstract The adenovirus E1A proteins are primarily concerned with modulating cellular metabolism to make the cell more susceptible to virus replication. In this study, adenovirus E1A gene was amplified via PCR on the HEK-293 cells genome and cloned into prokaryotic expression vector pRsetB. The recombinant plasmid pRsetB-E1A was ex- pressed i E.cOli BL21 and the relative molecular mass(Mr) of expressed fusion protein was approximately 36000. The recombinant protein was purified on a Ni-NIA agarose column and detected by SDSand Western blot. The purified recombinant protein was then injected into rabbits and anti-E1A polyclonal antibody with high titer was obtained. Keywords Adenovirus; E1A; Prokaryotic expression; Polyclonal antibody Article ID 1005-9040(2009)-06-891-04 1 lntroduction elements of viral replication, such as E1A and E1B[9,10].