低氧环境、雌二醇与睾酮.docVIP

低氧环境、雌二醇及睾酮 对红细胞的生成调节的影响 【摘 要】目的：观察低氧环境、雌二醇及睾酮对家兔红细胞的生成调节的影响。方法：选择健康的成年雄性与雌性家兔各16只分别分成四组，每组4只，分别为试验Ⅰ、Ⅱ、Ⅲ、Ⅳ。对试验Ⅰ组不给予任何处理，作为对照组；试验Ⅱ组用纱布分别捂住家兔的鼻腔与口腔持续30秒，制造低氧环境；试验Ⅲ组分别为家兔注射雌二醇1mg/kg；试验Ⅳ组分别为家兔注射丙酸睾酮1mg/kg。对每一组的家兔进行显微镜记数法测定红细胞数。结果表明：在正常情况下雄性家兔的红细胞数高于雌性家兔；在雄性家兔中，低氧刺激、注射丙酸睾酮过后，红细胞数分别增加了48.54%和53.89%，差异极显著（P0.01），注射雌二醇后，红细胞数略有减少，差异不显著（P0.05）；在雌性家兔中，低氧刺激、注射丙酸睾酮过后，红细胞数目分别增加了57.73%和53%，差异极显著（P0.01），注射雌二醇后，红细胞略有减少，差异不显著（P0.05）。结论：低氧刺激和注射丙酸睾酮能促进家兔红细胞生成，注射雌二醇能抑制家兔红细胞生成。 【关键词】红细胞；低氧环境；雌二醇；睾酮；生成调节 The Influence of Production Regulation on Red Blood Cells under the Hypoxia Environment, Estradiol and Testosterone College of Veterinary Medical of 2005 Huang shiyu instructor Hu tingxiu Abstract: Purchased grown-up male and female domestic rabbits 16 each in Sichuan agricultural market, observed two weeks, all rabbits were healthy, then we started experiments. Divided the 16 male and 16 female rabbits into four groups, separately, 4 for each group, named group Ⅰ,Ⅱ,Ⅲ and Ⅳ.In the group Ⅰ, we didn’t give any disposition, as the blank control group; In the group Ⅱ,we covered rabbits’ nasal cavities and the oral cavity with the gauzes for 30 seconds, to make hypoxia environment; In the group Ⅲ,we injected 1 mg of estradiol into each rabbit, respectively; In the group Ⅳ, we injected 1 mg of propionic acid testosterone into each rabbit, respectively. The number of red blood cells of each group were determined by traditional microscopic counting with counting plates. The results showed that the number of red blood cells in male rabbits was higher than the female rabbits. In the male rabbits, certain increase in red blood cells production were caused in the hypoxia environment group(groupⅡ) and in the injected propionic acid testosterone group(group Ⅳ), the increase rate was 48.54% and 53.89%, respectively, the difference was extremely remarkable(P0.01). In the female rabbits, certain increase in red blood cells production were caused in the hypoxia environment group (groupⅡ) and in th