抗浓核病毒中国株家蚕品系中肠羧酸酯酶活力变化和基因表达差异研究.docVIP

抗浓核病毒中国株家蚕品系中肠羧酸酯酶活力变化和基因表达差异的研究 高贵田1,2陈克平1姚勤1陈慧卿1王林玲1徐家萍1赵远1王永杰1 （1江苏大学生命科学研究院，镇江212013；2陕西师范大学食品工程系，西安710062）ctivity and Differential Display of Carboxylesterase in Bombyx mori Midgets of Against BmDNV-Z GAO Gui-tian1,2, CHEN Ke-ping1, YAO Qin1, CHEN Hui-qing1, WANG Lin-ling1, XU Jia-ping1, ZHAO Yuan1,WANG Yong-jie1 (1Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013；2Department of Food Enginneering,Shaanxi Normal University, Xian 710062) Abstract: 【Objective】 The study was used to discuss the relationship between the activity of Bombyx mori carboxylesterase (BmCarE) in the midgets. It was also needed to study the differential display of bmcare against Bombyx mori densonucleosis virus (BmDNV), and to depict the molecular mechanism of resistance to BmDNV. 【Method】 The activity of BmCarE in the midgets was detected by BIO-TEK SYNERGY after 12h, 36h and 72h inoculation with virus between the silkworm strain Huaba. This strain is susceptible to BmDNV-Z, and BC8 (a near isogenic line of Huaba),which is resistant to the same virus. The expression differential of the BmCar was investigated after 12h, 36h and 72h inoculation with virus by real-time fluorescence quantitative PCR. 【Result】 The activity of BmCarE in the midgets of BC8 inoculated with virus after 12h was 3.28, 2.26 and 3.02 times of that in the BC8 control, HUABA inoculated with virus and HUABA control respectively (P＜0.01)here was no statistic difference among other treatments; The relative quantity of BmCarE in the midgets of BC8 inoculated with virus after 12h was 17.714, 21.76 and 15.09 times of that in the BC8 control, Huaba inoculated with virus and Huaba control respectively (P＜0.01).here was no statistic difference among other treatments. 【Conclusion】 The increase of BmcarE activity and its expression level in the resistant strain inoculated after 12h may relate to the resistant gene (nsd/nsd) and the stimulation of BmDNV-Z in the resistant strain inoculated after 12h; The mo