雄黄纳米化后诱导人肺癌细胞株凋亡及抗血管生成体外地研究.pdfVIP

R ealgar N an ocry sta llin e in d u ced ap op tosis in h um an lun g can cer cell lin es an d its an ti-an giogen ic in v itro Sp eciality ； C lin ical R esearch of Integrated T C M and W estern M edicine A u th or ： L iu Z hai-dong T u tor ： P rofessor Q i Y uan-fu A B S T R A C T O bjectiv e : T o study th e tox ic com pound attack L iu shenw an in the m ain drugs realgar in nanoparticles and cisp latin-indu ced ap opto sis in cu ltured A 549 cells and anti-angiogen ic m echan ism ,then attack drug therap ies and treatm ent of lung can cer R e se a rch . M eth od s: H um an lung adenocarcinom a A 549 cells w ere cu ltured in v itro, th e tox ic com pound attack L iushenw an in the m ain drug realgar” nano-technology, w ith different concentration s of nanom eter realgar (25ug/m l, 50ug/m l，lOOug/m l ) and cisp latin (2ug/m l) alone or in com b ination w ith ，A 549 cells under the m icro scope and m orpho logical changes in the state ;A nn ex in V /P I doub le staining to detect cell ap opto sis; M T T assay inh ibition of cell proliferation ; Im m unoh istochem istry used to detect B cl-2, C aspase-3 expression ; R T -P C R assay Surviv in, c-M yc in the m R N A ; im m une staining integrinp l and E -cd ; im m unocytoch em istry m ethod p-caten in ; flow cytom etry detection of b -F G F ，M M P -9 expression . N anom eter realgar of A 549 hum an lun g can cer cells inh ib ited cell pro liferation ，induce ap optosis, anti-m etastasis and the p ossib le role of anti?angiogenesis m echan ism for attack ing dru g th erap ies based on the treatm ent of lung cancer prov ide the basis for th eory and laboratory evidence. R esu lts: D ifferent concentrations of nanom eter realgar can dam age hum an lun g cancer A 549 cells in the norm al form ; low concentration s to induce early apopto