反相色谱流动相的设计.ppt

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * So, let’s look at a comparison between the three different solid supports; we are using the same phase column for each in an effort to focus on just the effects that varying the solid support has on the observed LC 现在，我们来看看三个不同载体之间的比较；以上的结果是由相同的固定相及条件，但是不同载体所产出的色谱图。 Using the FP example at the top as the baseline, since it’s what most chromatographers are use to, when we move to core-shell particles we see a significant increase in plate count (22%), which translates into a 27% increase in resolution, however, we also see a significant increase in back pressure. 以最多色谱工作者使用的完全多孔颗粒结果为基准，当我们和核-壳颗粒比较时可以看到增加百分之22的平板记数，相等於百分之27的分离度，但是我们也会看到背压提升。 This increase in back pressure may be higher than you feel comfortable running at if using a 400 bar max pressure system. Additionally, this difference in back pressure is even greater if you are use to using 5 um columns 如果您是使用压力上限400巴的系统，您或许会觉得背压高於普通操作的水平，但如果您使用5微米的色谱柱，背压会上升的更多。 Looking at the Monolithic column, both efficiency and resolution achieved are the lowest of the three, but so is back pressure. In fact you are looking at a back pressure that is 1/3 that of the FP particle and 1/4 that of the core-shell particle. So, you can imagine how this can come in handy if you are looking to work with more viscous plasma samples. 当我们看到整体硅胶柱的结果，虽然柱效及分离度都是三者中最低的，但是背压也是最低的。 和前两个结果相比，您可以看到整体硅胶柱的压力是完全多孔性颗粒的1/3，核-壳颗粒的1/4。您应该不难想像，如果您打算分析黏稠的血浆样本，整体硅胶柱会是您最好的选择。 * * * * * * * * * * * * * * * * * * * * * * * Cationic selectivity, also known as silanol interactions, has gotten a bad rap over the years 阳离子选择性，或被称为硅醇基的交互作用，多年来一直不受欢迎。 Column manufacturers for years, PHE included, have gone to great lengths to eliminate this interaction and market to customers on how they have gotten rid of this interaction with different types of special end capping and super pure silica 在过去，包含Phenomenex在内的许多色谱柱制造商，竭尽所能的利用特别的不同端基封尾及超高纯度的硅胶以消除这个交互作用。 In the past, and today, where the problem has b