BulkAnalysis-OHLearning.pptVIP

W504 – Bulk Analysis Bulk Analysis - Introduction This session will not make you competent analysts! Aim is to make you aware of how samples are analysed and the limitations of the method Each asbestos type has slightly different chemical composition and crystal structure These differences are used to distinguish between the asbestos types (and between asbestos and other fibres) Bulk Analysis - Introduction Multi stage process starting with initial examination by eye, then under low power microscopy, leading to a tentative identification Some fibres that have been tentatively identified are examined using polarised light microscopy at high magnification to confirm fibre type Note: Polarised light microscopy is the most widely used method but other methods are available. Health and Safety Precautions Asbestos fibres need to be released from the sample matrix, so appropriate precautions must be in place All initial examination and sample preparation should be undertaken inside a safety cabinet Minimum face velocity of 0.5m/s. Fitted with a high efficiency “HEPA” filter. Visual indicator showing correct functioning Checked every day Tested every 6 months Risk assessments and precautions for working with acids, solvents and refractive index liquids Typical safety cabinet Initial Examination Entire sample examined by eye Initial assessment of possible type of asbestos product Visible fibres present or not? If sample non-homogenous each part examined Examination of sample using low power microscope Visible fibres present or not? Initial assessment of type(s) of fibre present Very fine fibres may still not be seen at this stage Appearance, colour and texture of any fibres noted and recorded Sample Preparation Purpose of sample preparation Ensure fibres released from sample matrix Remove fine particles adhering to fibres Obtain dry fibres (essential for dispersion staining) Sample preparation techniques include Physical extraction – tweezers, scraping of surface