Globalanalysisofpatternsofgeneexpressionduring.docVIP

Global analysis of patterns of gene expression during Drosophila embryogenesis Pavel Tomancak1,2,3,6, Benjamin P Berman1,2,7, Amy Beaton1,2,4, Richard Weiszmann4, Volker Hartenstein5, Susan E Celniker4 and Gerald M Rubin2,3,8 2Department of Molecular and Cell Biology, University of California, Berkeley, CA, 94720, 3Howard Hughes Medical Institute, 4Lawrence Berkeley National Laboratory, MS 64R0121, 1 Cyclotron Road, Berkeley, CA, 94720, 5Department of Molecular Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA90095, USA 6current address: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, Dresden, D-01307, Germany 7current address: Department of Preventive Medicine, Keck School of Medicine of USC, 1441 Eastlake Ave., Rm NOR 4423, Los Angeles CA, 90033 8current address: Janelia Farm Research Campus, HHMI, 19700 Helix Drive, Ashburn VA 20147 1Co-first authors Correspondence: Susan E. Celniker E-mail celniker@ Background: Cell and tissue specific gene expression is a defining feature of embryonic development in multi-cellular organisms.. However, the range of gene expression patterns, the extent of the correlation of gene expression with function, and the fraction and classes of genes whose spatial expression are tightly regulated, have been unclear due to the lack of an unbiased, genome-wide survey of gene expression patterns. Results:We have documented the embryonic expression of a representative sample of 6,003 (44%) of the 13,659 protein-coding genes identified in the Drosophila genome with 74,833 images and controlled vocabulary annotations, allowing us to make quantitative statements about gene expression patterns of the entire genome. Individual expression patterns are extraordinarily diverse, but by supplementing qualitative in situ data with quantitative microarray time-course data using a hybrid clustering strategy, we identify groups of genes with similar overall expression. 2,549 (57%) of