铁含量对小胶质细胞释放促炎因子影响庞小翼1,杨明2,张培根3(1,2,四川绵阳四0四医院麻醉科,绵阳621000,3四川绵阳四0四医院骨科,绵阳621000).docVIP

铁含量对小胶质细胞释放促炎因子影响 庞小翼1，杨明2，张培根3 （1，2，四川绵阳四0四医院麻醉科，绵阳621000，3四川绵阳四0四医院骨科，绵阳621000） 【摘要】目的 探索小胶质细胞内(microglia,MI)铁含量，对MI释放促炎因子的影响。方法 使用3,5,5—三甲基乙酰铁(3,5,5-trimethylhexanoyl,TMHF)或去铁胺（deferoxamine,DFO）改变大鼠离体脑MI内铁离子含量，使用ELISA检测上清液中IL-1β,TNF-α表达水平及核NF-κB表达数量在MI使用LPS激活前后的变化。结果 与对照组相比，LPS激活后MI释放促炎因子增多,并且在铁超载组进一步增多，反之，在铁剥夺组增多程度下降；核 NF-κB表达在MI被LPS激活后增加，但铁超载MI表达量没有进一步增加。结论 激活后MI释放促炎因子量明显受细胞内铁含量影响，但机制可能与NF-κB无关。 【关键词】小胶质细胞；铁；炎症 The effect of Cellular Iron Status on microglia release proinflammatory cytokines. Pang XiaoYi,Yang Ming,Zhang PeiGen Sichuan Mianyang 404 Hospital,Mianyang 621000,China. [Abstract] Objective To investigate cellular iron status influences the function of microglia release proinflammatory cytokines. Methods Microglia were incubated with 3,5,5-trimethylhexanoyl(TMHF) or deferoxamine(DFO) to change the cellular iron status.The levels of TNF-a,IL-1β in supernate fluid and the amount of nuclear NF-κB were detected by ELISA before and after induced by LPS. Results Compared with that of control microglia,LPS increases the proinflammatory cytokine release and the amounts of proinflammatory cytokines are enhanced if the microglia were initially iron loaded and diminished if the cells had been exposed to an iron chelator. LPS activation of the microglia significantly increased NF-kB activation, but the addition of iron did not increase the amount of activation. Conclusion the release of proinflammatory cytokines by microglia is affected by their iron status, but the production of cytokines by microglia may have no relation with the activation of NF-kB . [Key Words] microglia ; iron; inflammation 目前研究已经证实MI过度激活并释放大量炎症因子是阿尔茨海默病(Alzheimers Disease,AD), 帕金森病(Parkinson’s disease ,PD)等多种认知障碍类疾病发病重要环节。同时已有大量研究发现铁离子超载与认知障碍类疾病发病有重要联系。但是有关铁超载与小胶质细胞过度激活间的关系报道甚少，本实验利用TMHF，DFO建立离体铁超载与铁剥夺MI模型，观察了静止及LPS激活后MI在不同铁含量状态下释放促炎因子及核NF-κB表达情况，旨在发现铁超载与小胶质细胞激活的关系。 1.材料和方法 1.1.材料 DMEM培养基(美国PAA公司)， 中国武汉博士德生物工程公司)，Indianapolis公司），TMHF，DFO，LPS(Sigma公司) ，ELISA试剂盒(美国Biosource公司)等。 1.2.