水稻小粒密穗突变体sep1的遗传分析、基因定位与育种利用.doc

水稻小粒密穗突变体sep１的遗传分析、基因定位与育种利用 王跃星１,＃　吴昆２,＃　方云霞１　陈红旗１　倪深１　付向东２　朱旭东１, (１中国水稻研究所水稻生物学国家重点实验室,杭州３１０００６;２中国科学院遗传与发育生物学研究所,北京１００１０１;＃共同第一作 者;通讯联系人,EＧmail:ricezxd＠１２６．com) GeneticAnalysis,GeneMappingandBreedingApplicationofSmallGrainand DensePanicleMutantsep１inRice WANGYueＧxing１,＃,WUKun２,＃,FANGYunＧxia１,CHENHongＧqi１,NIShen１,FUXiangＧdong２, ZHUXuＧdong１, (１StateKeyLaboratoryofRiceBiology,ChinaNationalRiceResearchInstitute,Hangzhou３１０００６,China;２InstituteofGenetics andDevelopingBiology,ChineseAcademyofSciences,Beijing１００１０１,China;＃Theseauthorscontributedequallytothispaper; Correspondingauthor,EＧmail:ricezxd＠１２６．com) WANGYuexing,WUKun,FANGYunxia,etal．Geneticanalysis,genemappingandbreedingapplicationofsmall grainanddensepaniclemutantsep１inrice．ChinJRiceSci,２０１４,２８(３):２２９Ｇ２３４． Abstract:By６０CorＧrayradiation,asmallgrainanddensepaniclemutantsep１wasobtainedfromtheindicavariety Shuangkezao．ComparedtothewildtypeShuangkezao,itwasamutantwithsmallergrain,shorterspikeＧstalkbut moregrainnumberperpanicle．Thehistologicalsliceobservationofthemainstemshowedthatthemutanthadmore vascularbundlenumberinonecycleandmorecellnumberperunitareathanthewildtype．Geneticandallelism analysisresultsshowedthatthesmallgrainanddensepaniclemutantwascontrolledbyarecessivegene,anditwas differenttothedensepaniclegeneswhichhadbeenalreadycloned．Thenbyconstructingthegeneticsegregated populationandusingtheInDelmarkers,thesmallgrainanddensepaniclegenewasmappedonthecentromericregion ofchromosome５betweenmarkerXP２６Ｇ２８andXP２６Ｇ３１．Inaddition,thebreedingapplicationofthemutantwas carriedoutonCMSlinesandthestabilelinesofhighgenerationshadfinallybeenobtained． Keywords:rice;smallgrainanddensepanicle;geneticanalysis;genemapping;breedingapplication 王跃星,吴昆,方云霞,等．水稻小粒密穗突变体sep１的遗传分析、基因定位与育种利用．中国水稻科学,２０１４,２８ (３):２２９Ｇ２３４． 摘　要:通过６０Co衰变产生的r射线辐照籼稻品种“双科早”,获得小粒密穗突变体sep１,该突变体与野生型双科早相比表现 为籽粒变小,穗轴变短,着粒密度增加.主茎切片观察结果显示,突变体维管束数和单位面积细胞数均比野生型多.遗传分 析和等位性分析结果表明,该突变体为隐性突变,与已克隆的直立密穗基因不等位.利用InDel分子标记将小粒密穗基因 sep１定位在第５染色体着丝粒附近的两个标记XP２６Ｇ２８与XP２６Ｇ３１之间,物理距离约为３．８９６M