MeioticRecombination7.pptVIP

Meiotic Recombination Some particulars and a hypothesis of mine The Mechanism Overview The Double-stranded Break How do we know that recombination in yeast initiates with DSBs? Experiments show that Spo11 correlates to the DSB Spo11: Structure and function Reference: Topoisomerase RAD51: A homolog of RecA RAD50-MRE11 complex Reference: previous proposal of RAD50 dimer My hypothesis of meiotic recombination First, Spo11 proteins introduce negative supercoil to chromatin. Then RAD51 proteins bind on ssDNA of bubble structures. The homologic DNA strands pair and synapse, forming homo-tetra-stranded formations. The Structure is nicked in proper sites by a certain endonuclease, separate by the Synaptonemal complex formation, and yield the recombinates. a, Spo11+/+ mid leptotene nucleus (with γ-H2AX signal removed). DMC1 (RAD51 homolog in rat) foci are now abundant on axial elements. b, Spo11-/- mid leptotene nucleus. DMC1 is undetectable and the γ-H2AX signal remains very sparse. a, Spo11+/+ early pachytene nucleus (with γ?-H2AX signal removed). DMC1 foci (arrowheads) have disappeared from many but not all of the newly synapsed autosomal bivalents but remain on the largely asynapsed X chromosome (arrow). b, Same nucleus with the γ-H2AX signal, which is now restricted to the chromatin of the sex body. It is referred that RAD50 is suggested as ATP-modulated DNA crosslinker. So RAD50 may bring homologic DNA strands together with the conformation change stimulated by ATP hydrolyze. For there are bubble structures on the chromatin, the single strands may interact and anneal between different chromatids. Therefore, RAD51 proteins release from the DNA strands. In addition I presume that RAD52 and RAD54 et.al may act roles in forming the homo-tetra-stranded structure. * * Jack Szostak(1989) and colleagues found that the deletions of the region near the 5`-end of the ARG4 gene would reduce the meiotic conversion rate of this gene. They cloned a 15-kb fragment of DNA, incl