《Spectrophotometry》.pdfVIP

GE Healthcare Life Sciences Spectrophotometry Handbook Handbooks from GE Healthcare Life Sciences Imaging ?KTA Laboratory-scale Principles and Methods Chromatography Systems 29-0203-01 Instrument Management Handbook 29-0108-31 GST Gene Fusion System Handbook Gel Filtration 18-1157-58 Principles and Methods 18-1022-18 Affinity Chromatography Principles and Methods Recombinant Protein Purification 18-1022-29 Handbook Principles and Methods Antibody Purification 18-1142-75 Handbook 18-1037-46 Hydrophobic Interaction and Reversed Phase Chromatography Ion Exchange Chromatography and Principles and Methods Chromatofocusing 11-0012-69 Principles and Methods 11-0004-21 2-D Electrophoresis using immobilized pH gradients Cell Separation Media Principles and Methods Methodology and Applications 80-6429-60 18-1115-69 Microcarrier Cell Culture Purifying Challenging Proteins Principles and Methods Principles and Methods 18-1140-62 28-9095-31 Nucleic Acid Sample Preparation for Isolation of mononuclear cells Downstream Analyses Methodology and Applications Principles and Methods 18-1152-69 28-9624-00 High-throughput Process Development Western Blotting with PreDictor Plates Principles and Methods Principles and Methods 28-9998-97 28-9403-58 Strategies for Protein Purification Protein Sample Preparation Handbook Handbook 28-9833-31 28-9887-41 Contents Spectrophotometry basics 3 What is spectrophotometry 3 Definition 3 Lambert’s Law 4 Beer’s Law 4 Nucleic acid applications 6 Direct UV measurement 7 A260/A280 Ratio 7 A260/A230 Ratio 7 A320 Background correction 7 Nucleic acid measurements with low volume instruments 8 Protein applications 9 Protein concentration calculations 10 Christian and Warburg 10 Colorimetric methods 10 BCA 11 Biuret 11 Bradford 11 Lowry 11 2-D Quant Kit 11 Choosing your protein assay 12 Measuring proteins in low volume instruments 14 Other applications 15 Fluorescent dyes 15 Cell culture 15 Other molecules 16 Continued overleaf 1 Spectrophotometry