大孔吸附树脂纯化刺玫果总黄酮工艺研究论文.docVIP

摘 要 刺玫果学名野蔷薇,又名野刺玫果,山刺玫果,系蔷薇科蔷薇属植物山刺玫的成熟果实。广泛分布于我国的东北地区，尤以黑龙江省贮量最为丰富。现代药理学研究表明： 目前，总黄酮 本论文采用法刺玫果提取物中的总黄酮。探索洗脱剂、pH值、上样浓度等因素对过程的影响。采用紫外分光光度计法对总黄酮含量进行了分析，最终确定了洗脱剂的浓度7%、上样浓度为。该法简单，快捷，效率高，进一步拓展了刺玫果的应用领域和范围，为刺玫果的全面和综合利用提供科学的理论依据。 关键词：刺玫果总黄酮法 Abstract Rosa multiflorais is the scientific name of Rosa davurica pall，also known as Wild rosa fruit. Rosa mountain fruit,this fruit is the Rosa mountain fruit of Rosaceae genus Rosa.It is growing in east-north China, especially in province of Heilongjiang. Modern pharmaceutical research shows that Rosa davurica Pall contains rich nutrients and bioactive substances, anti-aging, prevention and treatment of cardiovascular disease, enhances immunity and the efficacy of nootropic, etc. Total flavonoids in the Rosa davurica Pall possess the function of prevention and treatment of high blood lipids, thrombosis dued to cardiovascular and cerebrovascular diseases. It has properties like high-efficiency and the advantages of low toxicity side effects.Thus it is worth in doing research on refining total flavonoids in the fruit. Recently, there are the several common methods in purifying Flavonoids such as water-extracting alcohol-precipitating,alcohol-extracting water-precipitating,acid-base method, saltdopositing,io-exchange and crystallization,and so on.In practice, there is the limit in all these method reported.Some new methods are investigated like flocculation precipitation, macroporous resin absorption,ultrafiltration,high-speed centrifugea- tion, and so on. 　 In this thesis, we report extraction of total flavonoids from Rosa davurica pall by using the method of Macroporous resin.It was investigated basing on how the type of Eluant,PH of solution,concentration of samples influences purification.Using Ultraviolet spectrophotometer method to analyze Flavonoids,This method is to know the content of total Flavonoids by measuring Ultraviolet absorption. As a result, it was found that the concentration of Eluant is 75 % ethanol, pH is 5