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论文标题 高效液相色谱法测定大黄中的大黄素、大黄酚的含量……………………………………………………………..………3 第一章：大黄的概述……………………………………………..……3 §1-1基本信息…………………………………………………......…3 §1-2化学成分……………………………………………………….. 4 §1-3药效作用……………………………………………...................4 1-3-1消化系统的影响……………………………………...….........4 1-3-2对血液系统的影响……………………………………….........4 1-3-3抗感染作用…………………………………………….………5 §1-4不良反应…………………………………………………………5 §1-5现代研究……………………………………………………………………6 §1-1仪器与试剂……………………………………………………….6 1-1-1仪器…………………………………………………………......6 1-1-1试剂…………………………………………………………......6 §2-2方法与结果…………………………………………….…..…. …7 2-2-1色谱条件………………………………………………………...7 2-2-2对照品的制备……………………………………………..…….7 2-2-3供试品的制备………………………………………………..….7 2-2-4阴性对照溶液的制备…………………………………………...7 2-2-5干扰试验…………………………………………………..…….7 2-2-6标准曲线的绘制……………………………………………….7 2-2-7精密度试验……………………………………………………………………………………………………………….7 2-2-9稳定性试验……………………………………………………….7 2-2-10加样回收率试验……………………………………………….8 2-2-11样品的测定…………………………………………………….8 第三章：讨论……………………………………………………………..8 §3-1药材含量测定中提取剂的确定……………………………….….8 §3-2HPLC法中检测波长的确定………………………………………HPLC法中线形范围的确定………………………………………9 参考文献……………………………………………………………………高效液相色谱法测定大黄中的大黄素、大黄酚的含量大黄是我国临床常用的中药之一，有“泻热通畅、凉血解毒、逐瘀通经、攻积导滞、利胆退黄”之功效，大黄中的有效成分有蒽醌类、芪类、苯丁酮类、鞣质类、色原酮类、萘类、有机酸、糖、蛋白质、甾醇等150多种成分。大量的研究表明，大黄的诸多制剂均以保存总蒽醌为目的。采用高效液相色谱法测定大黄中的大黄素、大黄酚的含量结果可靠。操作简单，结果可靠，与药典法没有显著性差异。高效液相色谱法大黄素The content: rhubarb is used clinically of traditional Chinese medicine in China, is one of the spilled hot unobstructed, cool blood detoxification, by stasis given.it, tapping product guide sluggish, cholagogue retreat yellow function, the efficient components have rhubarb anthraquinone kind, Qi kind, butyl benzene ketone, tannic acid kind, color original ketone, naphthalene class, organic acids, sugar, protein, such as phytosterols 150 multiple components. A number of studies, many preparations are in dahuang save total anthraquinone is a purpose. By HPLC rhubarb of emodin, rhubarb phenolic content and results are reliable. By HPLC big HuangZhong emodin and rhubarb phenol, the content of chromatographic column: Shim