不同发酵条件对Fusarium__省略_niZH0101产木聚糖酶影响_王权帅.pdfVIP

188 2011, Vol. 32, No. 13 食品科学 ※生物工程 不同发酵条件对Fusarium. solani ZH0101 产 木聚糖酶的影响 王权帅，康文丽，生吉萍，申 琳* ( 中国农业大学食品科学与营养工程学院，北京 100083) 摘 要：以本实验室筛选到的一株产木聚糖酶但不产纤维素酶的真菌Fusarium solani ZH0 10 1 为供试菌株，利用麦 秸为诱导物，对产木聚糖酶的液体发酵培养基进行优化。对发酵时间、麦秸添加量、培养基起始p H 值、磷酸 盐、金属离子、无机氮源和有机氮源对产酶的影响进行研究，优化最佳的培养条件。优化后的液体发酵培养基条 件为：麦秸20g/L 、KH2 P O4 4g/L 、CH3 COON H4 1g/L 、酵母膏2g/L 和起始pH 值为6 .0 ，发酵时间为14 d 。优化 条件下所产无纤维素酶活力的木聚糖酶酶活力为26 .85U/mL ，能比未优化前的20 .82U/mL 提高近30% 。 关键词：F usarium solani ZH0 10 1；木聚糖酶；液体发酵 Optimization of Fermentation Condition for Xylanase Production by Fusarium solani ZH0 101 WANG Quan-shuai ，KAN G Wen-li ，SHENG Ji-ping ，SHEN Lin * (College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) Abstract ：Cellulase-free xylanase has been widely applied in the food indu stry as well as the paper and pulp indu stry. Wheat straw as one of the most important agricultural by-products can be used to induce cellulase-free xylanase production through fermentation. In the present study, the optimal conditions of wheat straw fermentation by Fusarium solani ZH0 10 1 for producing cellulase-free xylanase were 20 g/L wheat straw, 4 g/L KH2PO4 , 1 g/L CH3COONH4 , 2 g/L yeast extract, initial pH of 6.0 and fermentation time of 14 days. Under the optimal fermentation conditions, the cellulase-free xylanase activity could reach up to 26.85 U/mL, which exhibited a 30% enhancement compared with the fermentation in original medium. Key words：Fusarium solani ZH0 10 1；xylan ase ；submerged fermentation 中图分类号：Q815 文献标识码：A 文章编号：1002-6630(20 11) 13-0 188-06