大孔吸附树脂纯化贯叶连翘提取物的研究.pdfVIP

大孔吸附树脂纯化贯叶连翘提取物的研究 雷雪， 杨云*，刘富岗， 王俊敏 （河南中医学院药学院，郑州 450008） 摘要：目的 优选纯化贯叶连翘提取物的大孔吸附树脂。方法 以金丝桃素为指标，考察AB-8、 S-8、CAD-45、D101、DA-201、DB-301、HPD-600、LSA-10、LSA-20、SP-825、XDA-6 共12种不同型号的大孔吸附树脂对贯叶连翘提取物的纯化效果。结果 所选出的LSA-10型 -1 大孔吸附树脂对金丝桃素的静态饱和吸附量可达1.9240mg·g ，静态解析率达98.9%，动 态比上样量为3.9083、比吸附量3.8540、比洗脱量2.8970 结论 LSA-10 型大孔吸附树脂 。 性能良好，可高效的纯化贯叶翘提取物。 关键词：贯叶连翘提取物；金丝桃素 ；大孔吸附树脂 PurificationofHypericumperforatumL.ExtractswithMacroporous PurificationofHypericumperforatumL.ExtractswithMacroporous PPuurriiffiiccaattiioonnooffHHyyppeerriiccuummppeerrffoorraattuummLL..EExxttrraaccttsswwiitthhMMaaccrrooppoorroouuss Resin Resin RReessiinn LEIXue,LIUFu-gang,YANGYun,WANGJunmin (((HenanUniversityofTraditionalChineseMedicine,Zhengzhou450008,China))( )) ABSTRACT: OBJECTIVE ABSTRACT: OBJECTIVE AABBSSTTRRAACCTT:: OOBBJJEECCTTIIVVEE To select the optimum macroporous resin for the purification of METRODS METRODS Hypericumextracts. MMEETTRROODDSS Taking hypericin as the index, and the purification effect of 12 typesofmacroporousresins,suchasAB-8、S-8、CAD-45、D101、DA-201、DB-301、HPD-600、 LSA-10、LSA-20、SP-825、XDA-6、860021, wereusedto investigating theextractsofHypericum Results Results perforatum. RReessuullttss The selected LSA-10 macroporous resin was determined as follow: the -1 staturated absorption capacity was 1.9240 mg·g , the desorption ratio was 98.9%; the dynamic sample content ratio was 3.9083, the absorption ratio was 3.8540, the elution content ratio was CONCLUTION CONCLUTION 2.8970. CCOONNCCLLUUTTIIOONN LSA-10 macroporous resin had an obvious better efficiency, which is moreeffectivefors