Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation.pdfVIP

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Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation.pdf

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Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation.pdf

Hindawi Publishing Corporation Journal of Skin Cancer Volume 2011, Article ID 936546, 8 pages doi:10.1155/2011/936546 Research Article Assessment of Transformed Properties In Vitro and of Tumorigenicity In Vivo in Primary Keratinocytes Cultured for Epidermal Sheet Transplantation ´ 1, 2 1 1 1 1 3 4 A. Thepot, A. Desanlis, E. Venet, L. Thivillier, V. Justin, A. P. Morel, F. DeFraipont, M. Till,5 V. Krutovskikh,2 M. Tommasino,2 O. Damour,1 and P. Hainaut2 1 Banque de Tissus et Cellules, Hopital E. Herriot, 69437 Lyon cedex 03, France ˆ 2 Centre International de Recherche sur le Cancer, 69372 Lyon cedex 08, France 3 Centre Leon Berard, 69373 Lyon cedex 03, France ´ ´ 4 Centre Hospitalier Universitaire, 38043 Grenoble cedex 09, France 5 Laboratoire de Cytogenetique HFME, 69677 Bron, France ´ ´ ´ Correspondence should be addressed to A. Thepot, thepot.amelie@ Received 30 June 2010; Accepted 28 August 2010 Academic Editor: Mark G. Lebwohl ´ Copyright © 2011 A. Thepot et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Epidermal keratinocytes are used as a cell source for autologous and allogenic cell transplant therapy for skin burns. The question addressed here is to determine whether the culture process may induce cellular, molecular