Gene Expression Profiling for In Silico Microdissection of Hodgkins Lymphoma Microenvironment and Identification of Prognostic Features.pdfVIP

Hindawi Publishing Corporation Advances in Hematology Volume 2011, Article ID 485310, 8 pages doi:10.1155/2011/485310 Review Article Gene Expression Profiling for In Silico Microdissection of Hodgkin’s Lymphoma Microenvironment and Identification of Prognostic Features Franc¸ois Bertucci,1, 2, 3 Bruno Chetaille,1 and Luc Xerri1, 3 1 Department of Bio-Pathology, Institut Paoli-Calmettes, 232, bd Sainte-Marguerite, 13273 Marseille Cedex 09, France 2 Department of Medical Oncology, Institut Paoli-Calmettes, 13009 Marseille, France 3 Faculty of Medicine, University of Mediterranee, 13284 Marseille Cedex 07, France Correspondence should be addressed to Luc Xerri, xerril@marseille.fnclcc.fr Received 2 August 2010; Accepted 11 November 2010 Academic Editor: Shaji Kumar Copyright © 2011 Franc¸ois Bertucci et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Gene expression profiling studies based on DNA microarrays have demonstrated their ability to define the interaction pathways between neoplastic and nonmalignant stromal cells in cancer tissues. During the past ten years, a number of approaches including microdissection have tried to resolve the variability in DNA microarray measurements stemming from cancer tissue sample heterogeneity. Another approach, designated as virtual or in silico microdissection, avoids the laborious and time-consuming step of anatomic microdissection. It consists of confronting the gene expression profiles of complex tissue samples to those of cell lines representative of different cell lineages, different differentiation stages, or different signaling pathways. This