Genome-Wide Analysis of the Yeast Transcriptome Upon Heat and Cold Shock.pdfVIP

Comparative and Functional Genomics Comp Funct Genom 2003; 4: 366–375. Published online 18 July 2003 in Wiley InterScience (). DOI: 10.1002/cfg.301 Primary Research Paper Genome-wide analysis of the yeast transcriptome upon heat and cold shock 1 ´ 1 ´ 1 ´ 1 2 M. Becerra , L. J. Lombardıa , M. I. Gonzalez-Siso , E. Rodrıguez-Belmonte , N. C. Hauser ´ 1 and M. E. Cerdan * 1 ´ ˜ ˜ Dpto. Biologıa Celular y Molecular, Universidad de La Coruna, F. Ciencias, Campus de La Zapateira s/n 15075, La Coruna, Spain 2Molecular-Genetic Genome Analysis Group, Deutsches Krebsforschungszentrum, Heidelberg, Germany *Correspondence to: Abstract ´ ´ M. E. Cerdan, Dpto. de Biologıa Celular y Molecular, F. Ciencias. DNA arrays were used to measure changes in transcript levels as yeast cells responded ˜ to temperature shocks. The number of genes upregulated by temperature shifts from Universidad de A Coruna, Campus de A Zapateira s/n A 30 ◦ C to 37 ◦ C or 45 ◦ C was correlated with the severity of the stress. Pre-adaptation ˜ of cells, by growth at 37 ◦ C previous to the 45 ◦ C shift, caused a decrease in the Coruna 15071, Spain. E-mail: bmanamrt@udc.es number of genes related to this response. Heat shock also caused downregulation of a set of genes related to metabolism, cell growth and division, transcription, ribosomal proteins, protein synthesis and destination. Probably all of these responses combine to slow down cell g