Genome-Wide Generation of Yeast Gene Deletion Strains.pdfVIP

Comparative and Functional Genomics Comp Funct Genom 2001; 2: 236–242. DOI: 10.1002/cfg.95 Review Genome-wide generation of yeast gene deletion strains Diane E. Kelly*, David C. Lamb and Steven L. Kelly Institute of Biological Sciences, University of Wales Aberystwyth, Aberystwyth, Wales, UK SY23 3DA Abstract *Correspondence to: D. E. Kelly, Institute of Biological In the year 2001 a collection of yeast strains will be completed that are deleted in the 6000 Sciences, University of Wales open reading frames selected as putative genes by the initial bioinformatic analysis of the Aberystwyth, Aberystwyth, Wales Saccharomyces cerevisiae genome. The collection was produced by the transatlantic yeast SY23 3DA, UK. gene deletion project, a collaboration involving researchers in the USA, Canada and E-mail: dek@aber.ac.uk Europe. The European effort was part of EUROFAN (European Functional Analysis Network) where some of the strains could feed into various functional analysis nodes dealing with specific areas of cell biology. With approximately 40% of human genes involved in heritable disease having a homologue in yeast and with the use of yeast in various drug discovery strategies, not least due to the dramatic increase in fungal infections, these strains will be valuable in trans-genomic studies and in specialised interest studies in individual laboratories. A detailed analysis of the project by the consortium is in Received: 26 June 2001