Highly Sensitive Detection of Organophosphate Insecticides Using Biosensors Based on Genetically Engineered Acetylcholinesterase and Poly(3,4-Ethylenedioxythiophene).pdfVIP

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Highly Sensitive Detection of Organophosphate Insecticides Using Biosensors Based on Genetically Engineered Acetylcholinesterase and Poly(3,4-Ethylenedioxythiophene).pdf

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Highly Sensitive Detection of Organophosphate Insecticides Using Biosensors Based on Genetically Engineered Acetylcholinesterase and Poly(3,4-Ethylenedioxythiophene).pdf

Hindawi Publishing Corporation Journal of Sensors Volume 2011, Article ID 102827, 7 pages doi:10.1155/2011/102827 Research Article Highly Sensitive Detection of Organophosphate Insecticides Using Biosensors Based on Genetically Engineered Acetylcholinesterase and Poly(3,4-Ethylenedioxythiophene) Tomasz Sikora,1 Georges Istamboulie,2 Elena Jubete,1 Estibalitz Ochoteco,1 Jean-Louis Marty,2 and Thierry Noguer2 1 New Materials Department, CIDETEC-Centre for Electrochemical Technologies, Parque Tecnologico de San Sebastian, ´ ´ Paseo Miramon 196, 20009 Donostia-San Sebastian, Spain ´ 2 Universite de Perpignan Via Domitia, IMAGES, Batiment S, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France ´ Correspondence should be addressed to Thierry Noguer, noguer@univ-perp.fr Received 14 September 2010; Accepted 24 January 2011 Academic Editor: Jiri Homola Copyright © 2011 Tomasz Sikora et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A poly(3,4-ethylenedioxythiophene) (PEDOT) conducting ink is presented as a new electroactive material to be incorporated in acetylcholinesterase-(AChE-) based screen printed biosensors, acting not only as a conducting template but also as an electrochemical mediator for thiocholine oxidation. Two diﬀerent strategies have been studied for the chemical synthesis of PEDOT: (a) a classical oxidative polymerisation and (b) a more innovative enzymatic polymerisation, giving a water-soluble PE