Integrating Genotypic Data with Transcriptomic and Proteomic Data.pdfVIP

Comparative and Functional Genomics Comp Funct Genom 2002; 3: 22–27. DOI: 10.1002/cfg.135 Conference Review Integrating genotypic data with transcriptomic and proteomic data Denis C. Shields* and Aisling M. O’Halloran Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, 123 St Stephen’s Green, Dublin 2, Ireland *Correspondence to: Abstract Department of Clinical Pharmacology, Royal College Historically genotypic variation has been detected at the phenotypic level, at the metabolic of Surgeons in Ireland, level, and at the protein chemistry level. Advances in technology have allowed its direct 123 St Stephen’s Green, visualisation at the level of DNA variation. Nevertheless, there is still an enormous interest Dublin 2, Ireland. in phenotypic, metabolic and protein property variability, since such variation gives E-mail: dshields@rcsi.ie insights into potential functionally important differences conferred by genetic variation. High-throughput transcriptomics and proteomics applied to different individuals drawn from a population has the potential to identify the functional consequences of genetic variability, in terms of either differences in expression of mRNA or in terms of differences in the quantities, pI(s) or molecular weight(s) of an expressed protein. Family studies can define the genetic component of such variation (segregation analysis) and with the geno- typing of well-spaced markers can map the causative factors to broad chromosomal regions (linkage analysis).