StepOne-gene荧光定量PCR.pdfVIP

StepOne™ Real-Time PCR System StepOne™ Real-Time PCR System ● Basics ● Installation Process 2 Basics StepOne™ Real-Time PCR System StepOne™ Real-Time PCR System: The Basics ● 3-color instrument – FAM™/SYBR® Green dyes – VIC®/JOE™ dyes – ROX™ dye – TAMRA™ dye is not recommended 4 StepOne™ Real-Time PCR System StepOne™ Real-Time PCR System: The Basics ●One block, Two speeds –Fast cycling: 40 cycles in under 40 minutes –Standard cycling: 40 cycles in under 2 hours – 48-wells – 10-30µl reaction volume 4 5 StepOne™ Real-Time PCR System StepOne™ Real-Time PCR System: The Basics ●Supported consumables: –48-well plates with optical adhesive cover –48-well plates with caps –Fast tubes –Fast 8-tube strips 6 StepOne™ Real-Time PCR System IMPORTANT: HOW TO LOAD THE BLOCK 7 StepOne™ Real-Time PCR System Basic Operation ● Soft power button on the LCD touchscreen 8 StepOne™ Real-Time PCR System Customer can use LCD touchscreen for EIGHT major activities 1. To upload a file from a USB stick 1. To upload a file from a USB stick 2. To download a completed file from the instrument to a 2. To download a completed file from the instrument to a USB stick USB stick 3. To configure shortcuts 4. To see progress in the thermal cycling protocol view only 5. To set up a thermal cycling profile for PCR or real-time PCR 6. To set the IP address of the instrument 7. To install the instrument 8. To prepare instrument for transportation 9 StepOne™ Real-Time PCR System Size Specifications 51.2 cm. (20.16 in.) 42.7 cm. (16.81 in.) 24.6cm. (9.69 in.) Weight: 23.6 kg. (52 lb.) 10 StepOne™ Real-Time PCR System Two Methods of Controlling the Instrumen