基因工程的主要技术原理创新.ppt

Agarose gel electrophoresis Gel electrophoresis is not only used as an analytical method, it is routinely used preparatively for the purification of specific DNA fragments. The gel is composed of: ? Agarose: for DNA fragments ranging in size from a few hundred base pairs to about 20 kb (Fig. 2.1). ? Polyacrylamide: for smaller DNA fragments. Agarose gel is a complex network of polymeric molecules whose average pore size depends on the buffer composition and the type and concentration of agarose used. ? In any event, gel electrophoresis is frequentlyperformed with marker DNA frag