黄孢原毛平革菌对土壤中五氯酚的降解.pdfVIP

中国农业科学 2004,37(4):577-583 ScientiaAgriculturaSinica 黄袍原毛平革菌对土壤中五氯酚的降解 吴 坤，徐淑霞，贾新成 (河南农业大学生物技术与食品科学学院，郑州 450002) 摘要:研究了白腐真菌典型种黄袍原毛平革菌 (尸hanerochaete chrysosporium)对土壤中五氮酚 (pentachlorophenol,PCP)的生物降解。结果表明，在灭菌土壤中PCP初始浓度为71.62mg.kg一干‘土条件下，P. chrysosporium生长和对PCP降解的最适接种量为。.10ml-g一干‘土，P.chrysosporlu。最适生长温度为370C，对 PCP降解的最适宜温度为30一37C，菌体生长和PCP降解的最适土壤含水量为25 。灭菌土壤中PCP的初始浓度为 50.05一175.72mg-kg一干‘土时，P.chrysosporiu，对PCP的降解符合一级动力学方程。在非灭菌土壤中PCP初始 浓度为67.50mg.kg一’干土条件下，PCP降解的最适接种量和温度分别为0.10MI-g-，干土和37C，但菌体生长的 最适接种量和温度分别为0.15ml-g-，干土和37C。最适条件下，P.chrysosporlum在 “d内对非灭菌土壤中PCP的 降解率达68.81%a 关键词:黄袍原毛平革菌;白腐真菌;五氮酚;生物降解;土壤修复 BiodegradationofPentachlorophenolinSoilby Phanerochaetechrysosporium WUKun,XUShu-xia,RAXin-cheng (CollegeofBiotechnologyandFoodScience,HenanAgriculturalUniversity,Zhengzhou450002) Abstract:Thebiodegradationofpentachlorophenol伊CP)-contaminatedsoilbytypicalwhiterotfungusPhanerochaete chrysosporiumwasstudied.TheresultsshowedthatwheninitialPCPconcentrationinsterilizedsoilwas71.62mg-kg-1dried soil,theidealconditionswere0.10ml-g-driedsoilofinoculationrate,25%ofsoilmoistureand370CforP.chrysosporium growth，andsameoffortheoptimumparametersforobtainingthemaximumdegradationratesofPCP.WhenPCPconcentration at50.05一175.72mg-kg-1driedsoilinsterilizedsoil,thedegradationconformedtoone-ratekineticequation.Inunsterilized soilwith67.50mg-kg-1driedsoilPCP,theoptimalinoculationratefordegradationofPCPandP.chrysosporiumgrowth were0.10ml-g-driedsoiland0.15ml-gdriedsoilrespectively.TheoptimaltemperatureforPCPdegradationandP. chrysosporiumgrowthwas37C.TherateofPCPdegradationwas68.81%wi