水溶性灵芝肽在动物体外的抗氧化活性.pdfVIP

中国农业科学 2006,39(12):2603-2607 Scientia Agricultura Sinica 水溶性灵芝肽在动物体外的抗氧化活性 何 慧，孙 颉，谢笔钧 （华中农业大学食品科技学院，武汉 430070 ） 摘要：【目的】研究水溶性灵芝肽在动物体外的抗氧化活性。【方法】用丙二醛(MDA)试剂盒测定 MDA 含量， 用分光光度法测定大鼠血清自氧化溶血及小鼠肝线粒体肿胀度。 【结果】当水溶性灵芝肽的剂量为 0.25 mg·ml-1 时，对大鼠红细胞自氧化溶血的抑制率达62.33%，对溶血过程中MDA生成的抑制率达91.24%；在有或无自由基诱 导剂（Fe2+ -1 或H O ）时，0.35 mg·ml 的水溶性灵芝肽均能极显著抑制小鼠肝匀浆中MDA 的生成（P ＜0.01），且抑制 2 2 率大致相当，为60%左右；当水溶性灵芝肽剂量为1.00 mg·ml-1 时，对线粒体肿胀度的抑制率为90.52%，对线粒体 中MDA生成的抑制率达65.42%，且呈明显剂量-效应关系。【结论】水溶性灵芝肽在体外具有明显的抗氧化作用。 关键词：灵芝; 肽; 抗氧化; 体外试验 The Antioxidative Activity of Water-Soluble Peptides from Ganoderma lucidum in vitro Test HE Hui, SUN Jie, XIE Bi-jun (College of Food Science Technology, Huazhong Agricultural University, Wuhan 430070) Abstract: 【Objective 】 The antioxidative activity of water-soluble peptides from Ganoderma lucidum (GLP) in vitro test was studied. 【Method 】 The reagent kits were used to determine the content of malondialdehyde (MDA). The auto-hemolysis of red blood cells (RBC) in rats and the swelling of rats liver mitochondria were observed by means of spectrophotometer. 【Result 】 The inhibiting rate of 0.25 mg·ml-1 GLP on the auto-hemolysis of rats RBC was 62.33% . The inhibiting rate on the MDA formation in rat RBC was 91.24%. 0.35 mg ml-1 GLP. It could prevent the MDA formation in mice liver homogenate (P0.01) with or without free radical inducers (Fe2+or H O ). They had approximate inhibiting rates around 60%. When 1.00 mg·ml-1 GLP was added, the 2 2 inhibiting rate on the swelling of mice liver mitochondria was 90.52%. The inhibiting rate on the MDA formation in mice liver