外源茉莉酸甲酯处理茶树对茶尺蠖幼虫生长的影响.pdfVIP

中国农业科学 2005,38(2):302-307 Scientia Agricultura Sinica 外源茉莉酸甲酯处理茶树对茶尺蠖幼虫生长的影响 1, 2 2 1 桂连友 , 陈宗懋 , 刘树生 1 2 （浙江大学应用昆虫学研究所，杭州 310029 ； 中国农业科学院茶叶研究所/农业部茶叶化学工程重点开放实验室，杭州 310008 ） 摘要：研究了应用外源茉莉酸甲酯处理茶树后，对茶树叶片中脂氧合酶、多酚氧化酶和蛋白酶抑制素活性， 以及对茶尺蠖幼虫中肠蛋白酶活性和生长的影响。结果表明，应用外源茉莉酸甲酯喷雾法或暴露法处理茶树，都 能够诱导茶树叶片脂氧合酶、多酚氧化酶和蛋白酶抑制素活性增加，茶尺蠖幼虫中肠强碱性类胰蛋白酶活性和类 胰凝乳蛋白酶活性受抑制，但弱碱性类胰蛋白酶活性未受抑制，从而导致茶尺蠖幼虫中肠内各种蛋白酶间的协调 性遭到破坏，生长受阻，这可能是茉莉酸甲酯诱导茶树对茶尺蠖幼虫产生直接抗性作用的一种机制。 关键词：茉莉酸甲酯; 蛋白酶抑制素; 中肠蛋白酶; 茶尺蠖; 茶树 Effect of Exogenous MJA Treatment of Tea Plants on the Growth of Geometrid Larvae 1, 2 2 1 GUI Lian-you , CHEN Zong-mao , LIU Shu-sheng 1 2 ( Institute of Applied Entomology, Zhejiang University, Hangzhou 310029; Key Laboratory of Tea Chemical Engineering, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008) Abstract: The effect of tea plants Camellia sinensis induced by exogenous methyl jasmonate (MJA) on lipoxygenase (LOX), polyphenol oxidase (PPO) and proteinase inhibitor (PI) activity in the leaves of tea plants, as well as the growth and midgut proteinase activity of geometrid Ectropis obliqua larvae were studied. MJA significantly induced LOX, PPO and PI activity in tea leaves of tea plants. When geometrid larvae have fed on leaves of tea plants treated with MJA, the activities of the high alkaline trypsin-like enzyme and chymotrypsin-like enzyme in their midgut were significantly inhibited, but the activities of the low alkaline t