温度对隐性长穗颈温敏不育水稻eui基因表达的影响.pdfVIP

中国农业科学 2005,38(2):222-227 Scientia Agriucultura Sinica 温度对隐性长穗颈温敏不育水稻 eui 基因表达的影响 1，2 1 1 1 1 肖辉海 ，钟卫华，梁满中，徐孟亮，陈良碧 1 2 （湖南师范大学生命科学学院，长沙 410081 ； 湖南农业大学理学院，长沙 410182 ） 摘要：为了解杂交稻不育系抽穗不畅问题，以隐性长穗颈温敏核不育水稻长选 3S 为材料，采用田间调查和 人工温度处理方法研究了温度对隐性长穗颈基因（eui ）表达的影响。结果表明，长选 3S 穗颈伸出剑叶叶鞘的长 度与始花前9～20 d自然条件下的日均温度呈负相关，其中以始花前12～17 d(花粉母细胞形成至减数分裂期)日 均温度负相关性最显著。在 eui 基因表达对温度最敏感的时期进行4种人工温度处理，22℃、24℃、26℃和28℃。 28℃条件下 eui 基因表达受阻；22～26℃条件下 eui 基因表达，且穗颈伸出剑叶叶鞘的长度随温度降低而增长。 关键词：水稻；温敏核不育水稻；隐性长穗颈；温度；相关性 Effect of Temperature on the eui Gene Expression of TGMS Rice 1,2 1 1 1 1 XIAO Hui-hai , ZHONG Wei-hua , LIANG Man-zhong , XU Meng-liang , CHEN Liang-bi 1 ( College of Life Science, Hunan Normal University, Changsha 410081 ; 2College of Sciences, Hunan Agricultural University, Changsha 410182 ) Abstract: Effect of temperature on the eui gene expression of TGMS rice Changxuan 3S were analyzed under the condition of natural ecology and different artificial temperature. The results were as following: There was a negative correlation between panicle elongation and natural temperature at 9-20 days before flowering, and the negative correlation of the panicle elongation with mean diurnal temperature at 12-17 days (from pollen mother cell formation to meiosis) before flowering was the most significant. In the thermo-sensitive period of the eui gene, when Changxuan 3S was treated with the condition of constant 22 ℃,24 ℃,26 ℃,28 ℃, the eui gene could express under the treatment of 22 ℃, 24 ℃, 26℃, respectively, and the lower the temperature treated, t