Effects of Simulated Weightlessness on Tight Junction Protein Occludin and Zonula Occluden-1 Expression Levels in the Intestinal Mucosa of Rats.pdfVIP

Effects of Simulated Weightlessness on Tight Junction Protein Occludin and Zonula Occluden-1 Expression Levels in the Intestinal Mucosa of Rats.pdf

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Effects of Simulated Weightlessness on Tight Junction Protein Occludin and Zonula Occluden-1 Expression Levels in the Intestinal Mucosa of Rats.pdf

JHuazhongUnivSciTechno1LMedSciJ DOI10.1007/sll5960l1.0145—5 31(1):2632,201l 26 Effects of Simulated W eightlessness on Tight Junction Protein Occludin andZonulaOccluden一1ExpressionLevelsintheIntestinal M ucosaofRats YingCHEN(陈 英)一,ChunminYANG(杨春敏),QingsenLIU (刘庆森),MingzhouGUO(郭明洲), YunshengYANG(杨云生),GappingMAO(毛高平),PingWANG(王 萍) lPLAPostgraduateMedicalSchool , Beijing100853.China 2DepartmentofGastroenterologyandResearchCenterofAeropathy,GeneralHospitalofAirForce.PLA.Bejiing100036,China 3DepartmentofGastroenterology,GeneralHospitalofPLA.Bejiing100853.China ◎HuazhongUniversityofScienceandTechnologyandSpringer-VerlagBerlinHeidelberg2011 Summary:Thisstudyinvestigatedthetightiunction(TJ)proteinexpressionoftheintestinalmucosain arattail—suspensionmodelundersimulatedweightlessness.Twenty—fourW ~starratswererandomly dividedintothreegroups:CONgroup =8),control;SUS一14dgroup( 8),tail—suspensionfor14days; SUS.21dgroupf”=81,tail.suspensionofr21days.OccludinandZonulaOceluden.1(Z0—1)expression levelsweredeterminedby immunohistochemicalanalysisandmRNA fluorescentquantitativePCR. Plasma levels ofdiamine oxidase (DAO) and d—lactatewere determined using enzymatic spectrophotometry.ImmunohistochemicalresultsforoccludinandZ0—1showeddisurptionoftheTJsin theintestinalmucosainSUS.14dandSUS一21dgroups.TheexpressionlevelsofoccludinandZO.1in SUS一21dgroupwerelowerthanthoseinSUS.14dgroup andsignificantlylowerthanthoseinC0N groupf0ccldin:0.86~0.02vs1.01-40.03vs1.634-0.03andZ0.1:0.82~0.01vs1.00~0.02vs1.55~0.01.

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