抑制玉米幼胚愈伤组织褐化的研究_宋钦虎.pdfVIP

农学学报 2012,2(05):24-27 Journal of Agriculture 抑制玉米幼胚愈伤组织褐化的研究 宋钦虎，郭新梅，裴玉贺，宋希云 （青岛农业大学农学与植物保护学院/青岛市主要农作物种质创新与应用实验室，山东青岛266109 ） 摘 要：褐化是植物组织培养中一种常见的问题，直接影响组织培养能否成功。为了控制植物组织培养 中的褐化现象，选取3 个玉米自交系丹黄25、P 138、H21 的幼胚为外植体，着重探讨胚龄，2,4-D、甘露醇、 L-脯氨酸和AgNO 浓度对玉米幼胚愈伤组织褐化的影响。结果表明，选取授粉11天的‘丹黄25 ’幼胚、 3 13天的P 138和H21 幼胚接种到诱导培养基中，待长出愈伤后转移到添加2 mg/L 2,4-D、10 g/L 甘露醇、 10 mg/L L-脯氨酸、10 mg/L 硝酸银的继代培养基上，愈伤组织褐化率显著降低，表明培养基的种类及添 加物对愈伤组织的生长有重要影响。 关键词：玉米幼胚；愈伤组织；组织培养；褐化 中图分类号：S513 文献标志码：A 论文编号：2011-0939 Primary Study on Reducing Browning of Callus Induced from Maize Immature Embryo SongQinhu,GuoXinmei,PeiYuhe,SongXiyun (QindaoKeyLabofGermplasmInnovationandApplicationofMajorCrops/CollegeofAgronomyand PlantProtectionQingdaoAgriculturalUniversity,Qingdao266109,Shandong,China) Abstract:Materialbrowningwasauniversalphenomenoninplant-tissueculture,anditplayavitalrolein plant-tissueculture.Tocontrolthebrowingofplant-tissueculture,theimmatureembryosofthreemaize inbred lines (Danhuang 25, P138, H21) were used in the experiment. Effect of embryo age, 2,4-D concentration,mannitolconcentration,L-prolineandsilvernitrateconcentrationonthebrowningofmaizecalli were studied. The results showed that the embryos selected from ‘Danhuang 25’(11-day after self-pollination),P138andH21 (13-dayafterself-pollination)wereinoculatedintoinductionmediumand thentheircallusbrowningcouldbesignificantlyinhibitedbyadditionof2 mg/L 2, 4-D, 10g/Lmannitol, 10 mg/L L-proline, 10 mg/Lsilvernitrateinsubculturemedium,indicatedthatthetypeofmediumand additivedhaveamajorimpactoncallusgrowth. Key words:MaizeImmatureEmbryo;Callus;TissueCulture;Browning 0 引言