PTV_GC_MS_MS同时测定烟草中202种农药残留.pdfVIP

烟草科技/TobaccoScienceTechnology 烟草化学/Tobacco Chemistry 2013年第 8期（总第313期） PTV-GC-MS/MS同时测定烟草中202种农药残留 1 1 1 2 2 2 1 楼小华 ，高川川 ，朱文静 ，张洪非 ，唐纲岭 ，边照阳 ，巴金莎 1. 贵州省烟草质量监督检测站，贵阳市金阳新区云潭北路 550025 2. 国家烟草质量监督检验中心，郑州高新技术产业开发区枫杨街2号 450001 摘要：研究了应用程序升温汽化进样-气相色谱-串联质谱（PTV-GC-MS/MS ）技术检测烟草中202 种农药 品种221 个组分的高效方法。以QuEChERS 技术快速提取烟草中残留农药并净化，PTV 进样后在 TR-pesticide II 毛细管柱上分离，通过优化串联质谱参数，有效降低复杂基质干扰及农药组分重叠峰的相 互串扰，以保留时间窗口和特征SRM （选择性反应监测）离子对定性、峰面积定量。结果表明：①202 种农 药在0.01~2.50 mg/L 范围内线性良好，相关系数为0.9800~0.9999 ，检测限0.0002~0.0100 mg/kg ，加标回收 率67.4%~ 112.0% ，RSD 0.30%~20.30% 。②应用该方法对44 个进口烟叶样品进行检测验证，效果良好。③ 该方法对农药品种覆盖面广，前处理简便、快捷，节省溶剂；一次进样在46 min 内可分析221 个组分，准确、 灵敏。 关键词：程序升温汽化（PTV ）；气相色谱-串联质谱；烟草；农药残留 中图分类号：TS411.2 文献标识码：B 文章编号：1002-0861 （2013 ）08-0045-13 SimultaneousDeterminationof 202 PesticideResiduesinTobaccobyPTV-Gas Chromatography- TandemMassSpectrometry 1 1 1 2 2 2 LOU Xiaohua , GAO Chuanchuan , ZHU Wenjing , ZHANG Hongfei , TANG Gangling , BIAN Zhaoyang , and BA Jinsha1 1. Guizhou Tobacco Quality Supervision Test Station, Guiyang 550025, China 2. China National Tobacco Quality Supervision Test Center, Zhengzhou 450001, China Abstract: An effective method for detecting 202 pesticide residues (221 components) in tobacco was developed by PTV-GC-MS/MS (Programmed temperature vaporization sampling technology-gas chromatography-tandem mass spectrometry). The pesticide residues in tobacco sample were extracted rapidly and purified with QuEChERS technique, then separated on TR-pesticideII capillary chromatographic column after PTV sampling. The interference of complex matrix and peak overlapping of various pesticide components were reduced by means of optimizing tandem mass spectrometry parameters. Qualitat