Genome sequencing reveals agronomically important loci in rice.pdfVIP

A rt i c l e s Genome sequencing reveals agronomically important loci in rice using MutMap 1,2,7 3,7 3 3 2,3 3 Akira Abe , Shunichi Kosugi , Kentaro Yoshida , Satoshi Natsume , Hiroki Takagi , Hiroyuki Kanzaki , 3,4 3 3 3 5 6 Hideo Matsumura , Kakoto Yoshida , Chikako Mitsuoka , Muluneh Tamiru , Hideki Innan , Liliana Cano , 6 3 Sophien Kamoun Ryohei Terauchi The majority of agronomic traits are controlled by multiple genes that cause minor phenotypic effects, making the identification . d of these genes difficult. Here we introduce MutMap, a method based on whole-genome resequencing of pooled DNA from a e v segregating population of plants that show a useful phenotype. In MutMap, a mutant is crossed directly to the original wild-type r e s line and then selfed, allowing unequivocal segregation in second filial generation (F ) progeny of subtle phenotypic differences. e 2 r This approach is particularly amenable to crop species because it minimizes the number of genetic crosses (n = 1 or 0) and s t mutant F progeny that are required. We applied MutMap to seven mutants of a Japanese elite rice cultivar and identified the h 2 g i unique genomic positions most probable to harbor mutations causing pa